Recombinant Environmental Libraries Provide Access to Microbial Diversity for Drug Discovery from Natural Products (Record no. 43444)
[ view plain ]
| 000 -LEADER | |
|---|---|
| fixed length control field | 02448nam a2200349Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625124734.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-9188 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | Recombinant Environmental Libraries Provide Access to Microbial Diversity for Drug Discovery from Natural Products |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | Applied and Environmental MicroBiology, 69, p.49-55, 2003 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | To further explore possible avenues for accessing microbial biodiversity for drug discovery from natural products, we constructed and screened a 5,000-clone "shotgun" environmental DNA library by using an Escherichia coli-Streptomyces lividans shuttle cosmid vector and DNA inserts from microbes derived directly (without cultivation)from soil. The library was analyzed by several means to assess diversity, genetic content, and expression of heterologous genes in both expression hosts. We found that the phylogenetic content of the DNA library was extremely diverse, representing mostly microorganisms that have not been described previously. The library was screened by PCR for sequences similar to parts of type I polyketide synthase genes and tested for the expression of new molecules by screening of live colonies and cell extracts. The results revealed new polyketide synthase genes in at least eight clones. In addition, at least five additional clones were confirmed by high-pressure liquid chromatography analysis and/or biological activity to produce heterologous molecules. These data reinforce the idea that exploiting previously unknown or uncultivated microorganisms for the discovery of novel natural products has potential value and, most importantly, suggest a strategy for developing this technology into a realistic and effective drug discovery tool. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Courtois, S. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Cappellano, C.M. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Ball, M. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Francou, F.X. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Norman, F. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Helynck, G. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Martinez, A. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Martinez, A. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Hopke, J. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Osburne, M.S. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | August, P.R. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Nalin, R. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Guerineau, M. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Jeannin, P. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Simonet, P. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Pernodet, J.L. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1B6fPoNHj7vns2MCPtjI_I3RA3uM_9JZy/view?usp=drivesdk">https://drive.google.com/file/d/1B6fPoNHj7vns2MCPtjI_I3RA3uM_9JZy/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-9188 | 25.06.2025 | 25.06.2025 | Documentos solicitados |