Sequence-based approach for identification of cell wall proteins in Saccharomyces cerevisiae (Record no. 43475)

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control field 20250625140608.0
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Transcribing agency CICY
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Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-9220
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Title Sequence-based approach for identification of cell wall proteins in Saccharomyces cerevisiae
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Volume/sequential designation Current Genetics, 5(40), p.311-316, 2002
520 3# - SUMMARY, ETC.
Summary, etc. Open reading frames (ORFs)in the genome of Saccharomyces cerevisiae were screened for cell wall proteins and extracellular proteins, using an in silico sequence analysis combined with biochemical examination. The selection criteria used in the sequence analysis were the presence of a signal sequence for secretion and the absence of any targeting and retention signal to/in intracellular components. By using the PSORT II program, 163 ORFs/proteins were selected as potential extracellular proteins, including cell wall proteins. Of these, 51 ORFs/proteins of unknown localization and more than 120 amino acids in size were further studied on their cellular localization. A hemagglutinin (HA)epitope was inserted in the most C-terminus of each protein and the resulting HA-tagged protein was expressed under the authentic promoter in yeast cells. Out of the 51 constructs, 35 gave protein bands on Western blots. Examination of proteins in fractionated samples identified 11 extracellular proteins; six proteins that were weakly associated with the cell wall and five proteins that were relatively tightly associated with the cell wall.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element SACCHAROMYCES CEREVISIAE
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Topical term or geographic name entry element EXTRACELLULAR PROTEINS
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Topical term or geographic name entry element CELL WALL PROTEINS
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element IN SILICO SEQUENCE ANALYSIS
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Personal name Terashima, H.
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Personal name Fukuchi, S.
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Personal name Nakai, K.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Arisawa, M.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Hamada, K.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Yabuki, N.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Kitada, K.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1RBU51EB_2Nhm6TAV0npB0paVlilrc7j5/view?usp=drivesdk">https://drive.google.com/file/d/1RBU51EB_2Nhm6TAV0npB0paVlilrc7j5/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-9220 25.06.2025 25.06.2025 Documentos solicitados