Birch PR-10c interacts with several biologically important ligands (Record no. 43506)

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fixed length control field 02488nam a2200349Ia 4500
003 - CONTROL NUMBER IDENTIFIER
control field MX-MdCICY
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250625140609.0
040 ## - CATALOGING SOURCE
Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-9251
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
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245 10 - TITLE STATEMENT
Title Birch PR-10c interacts with several biologically important ligands
490 0# - SERIES STATEMENT
Volume/sequential designation PhytoChemistry, 66(21), p.2524-2533, 2005
520 3# - SUMMARY, ETC.
Summary, etc. PR-10c is a unique member of PR-10 proteins in birch, since it is the only one known to be post-translationally modified by glutathione and is not constitutively expressed in pollen. Both reduced and S-glutathiolated forms of PR-10c show low ribonuclease activity. However, the major function of the protein is apparently not yet resolved. Our protein-ligand interaction studies with saturation transfer difference (STD)NMR revealed that PR-10c interacts with several biologically important molecules, including cytokinin, flavonoid glycosides, sterols and emodin. Competition study with deoxycholate and kinetin revealed no statistically significant binding interference, indicating that these ligands have different binding sites in PR-10c. Ligand docking studies with a molecular model of PR-10c support the STD NMR results of ligand binding and binding epitopes, suggesting that there are three potential binding sites in PR-10c: two in the hydrophobic cavity and one in the glycine-rich loop. Our docking calculations suggested that only kinetin interacts with the glycine-rich loop, the binding occurring through its adenine moiety. Clear ligand specificity could be observed in the binding of nucleotide derivatives. S-glutathiolation of PR-10c did not affect kinetin binding. The present results suggest that birch PR-10c is a multifunctional protein, which has diverse roles in plant stress responses.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element SATURATION TRANSFER DIFFERENCE NMR
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element MOLECULAR MODELLING
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element PATHOGENESIS-RELATED PROTEINS
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element GLUTATHIOLATION
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element CYTOKININS
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element PHYTOSTEROLS
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element FLAVONOIDS
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element EMODIN
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Koistinen, K.M.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Soininen, P.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Venalainen, T.A:
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Hayrinen, J.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Laatikainen, R.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Perakyla, M.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Tervahauta, A.I.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Tervahauta, A.I.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/123F2dVyCSMxc3Rb-Gvq5oO2MORW9yu_G/view?usp=drive_link">https://drive.google.com/file/d/123F2dVyCSMxc3Rb-Gvq5oO2MORW9yu_G/view?usp=drive_link</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Source of classification or shelving scheme Clasificación local
Koha item type Documentos solicitados
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-9251 25.06.2025 25.06.2025 Documentos solicitados