Rapid large scale purification of ellagitannins from pomegranate husk, a by-product of the commercial juice industry (Record no. 43522)

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control field 20250625140609.0
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Transcribing agency CICY
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Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-9267
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245 10 - TITLE STATEMENT
Title Rapid large scale purification of ellagitannins from pomegranate husk, a by-product of the commercial juice industry
490 0# - SERIES STATEMENT
Volume/sequential designation Separation and Purification Technology, 41(1), p.49-55, 2005
520 3# - SUMMARY, ETC.
Summary, etc. Pomegranate (Punica granatum L.)fruits are widely consumed fresh and in processed forms as juice, jams and wine. Pomegranate fruit husk/peel is a rich source of hydrolyzable tannins called ellagitannins (ETs). In the commercial pomegranate juice (PJ)industry, these ETs are extracted from the husk in significant quantities into the juice due to their hydrophilic properties. Pomegranate husk, a by-product of the PJ industry, is therefore an inexpensive and abundant source of ETs. Previous methods to isolate pomegranate ETs included labor intensive and time-consuming solid phase extractions by column chromatography (C-18, polyamides, cellulose, Sephadex Lipophilic LH-20, Diaion HP20)and/or use of specialized instruments such as preparative-high performance liquid chromatography (HPLC). We have used an Amberlite XAD-16 resin vacuum-aspirated column to rapidly purify an aqueous extract of pomegranate husk to afford total pomegranate tannins (TPT)in substantial yields (58-60 g TPT/kg husk; time <1 h). Using analytical HPLC and tandem LC-ES/MS, evaluation of TPT showed that it contains the major fruit husk ET, punicalagin
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element POMEGRANATE
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element PUNICA GRANATUM
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element ELLAGITANNINS
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element PUNICALAGIN
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element AMBERLITE XAD-16
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Seeram, N.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Lee, R.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Hardy, M.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Heber, D.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1VzL_QsyNU8g5acNfYziYKfAQBTFqdeq4/view?usp=drivesdk">https://drive.google.com/file/d/1VzL_QsyNU8g5acNfYziYKfAQBTFqdeq4/view?usp=drivesdk</a>
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