The Plant Defense Elicitor Cryptogein Stimulates Clathrin-Mediated Endocytosis Correlated with Reactive Oxygen Species Production in Bright Yellow-2 Tobacco Cells1[C] (Record no. 43538)

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control field 20250625140609.0
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Transcribing agency CICY
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Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-9286
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Title The Plant Defense Elicitor Cryptogein Stimulates Clathrin-Mediated Endocytosis Correlated with Reactive Oxygen Species Production in Bright Yellow-2 Tobacco Cells1[C]
490 0# - SERIES STATEMENT
Volume/sequential designation Plant Physiology, 146(3), p.1255-1266, 2008
520 3# - SUMMARY, ETC.
Summary, etc. The plant defense elicitor cryptogein triggers well-known biochemical events of early signal transduction at the plasma membrane of tobacco (Nicotiana tabacum)cells, but microscopic observations of cell responses related to these early events were lacking. We determined that internalization of the lipophilic dye FM4-64, which is a marker of endocytosis, is stimulated a few minutes after addition of cryptogein to tobacco Bright Yellow-2 (BY-2)cells. This stimulation is specific to the signal transduction pathway elicited by cryptogein because a lipid transfer protein, which binds to the same receptor as cryptogein but without triggering signaling, does not increase endocytosis. To define the nature of the stimulated endocytosis, we quantified clathrin-coated pits (CCPs)forming on the plasma membrane of BY-2 cells. A transitory stimulation of this morphological event by cryptogein occurs within the first 15 min. In the presence of cryptogein, increases in both FM4-64 internalization and clathrin-mediated endocytosis are specifically blocked upon treatment with 5 mM tyrphostin A23, a receptor-mediated endocytosis inhibitor. The kinetics of the transient increase in CCPs at the plasma membrane coincides with that of transitory reactive oxygen species (ROS)production occurring within the first 15 min after elicitation. Moreover, in BY-2 cells expressing NtrbohD antisense cDNA, which are unable to produce ROS when treated with cryptogein, the CCP stimulation is inhibited. These results indicate that the very early endocytic process induced by cryptogein in tobacco is due, at least partly, to clathrin-mediated endocytosis and is dependent on ROS production by the NADPH oxidase NtrbohD.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Leborgne-Castel, N.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Lherminier, J.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Der, C.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Fromentin, J.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Houot, V.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Simon-Plas, F.
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Uniform Resource Identifier <a href="https://drive.google.com/file/d/1Pk_osK4iB3FZlqaNxjvnHduxTS1kHu1d/view?usp=drivesdk">https://drive.google.com/file/d/1Pk_osK4iB3FZlqaNxjvnHduxTS1kHu1d/view?usp=drivesdk</a>
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Source of classification or shelving scheme Clasificación local
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-9286 25.06.2025 25.06.2025 Documentos solicitados