Identification of a novel 4-hydroxyphenylpyruvate dioxygenase from the soil metagenome (Record no. 43590)
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| fixed length control field | 01978nam a2200253Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625140610.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-9340 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | Identification of a novel 4-hydroxyphenylpyruvate dioxygenase from the soil metagenome |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | BioChemical and Biophysical Research Communications, 31(370), p.322-326, 2008 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | 4-Hydroxyphenylpyruvate dioxygenase (HPPD)is a Fe(II)-dependent, non-heme oxygenase that converts 4-hydroxyphenylpyruvate to homogentisate. Essential cofactors, such as plastoquinone and tocopherol, are produced by HPPD-dependent anabolic pathways in plants. To isolate a novel hppd using cultureindependent method, a cosmid metagenomic library was constructed from soil in Korea. Screening of Escherichia coli metagenomic libraries led to the identification of a positive clone, YS103B, producing dark brown pigment in Luria-Bertani medium supplemented with L-tyrosine. In vitro transposon mutagenesis of YS103B showed that the 1.3 kb insert was sufficient to produce the hemolytic brown pigment. Sequence analysis of YS103B disclosed one open reading frame encoding a 41.4 kDa protein with the well-conserved prokaryotic oxygenase motif of the HPPD family of enzymes. The HPPD-specific b-triketone herbicide, sulcotrione, inhibited YS103B igmentation. The recombinant protein expressed in E. coli generated homogentisic acid. Thus, we present the successful heterologous expression of a reviously uncharacterized hppd gene from an uncultured soil bacterium. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Lee, C.M. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Yeo, Y.S. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Lee, J.H. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Kim, S.J. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Kim, J.B. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Han, N.S. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Koo, B.S. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Koo, B.S. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1qWw-BdFw8zQiqUJdMfzzGNYecL7u_QMj/view?usp=drivesdk">https://drive.google.com/file/d/1qWw-BdFw8zQiqUJdMfzzGNYecL7u_QMj/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-9340 | 25.06.2025 | 25.06.2025 | Documentos solicitados |