Isolation and characterisation of Gaeumannomyces graminis var. graminis melanin mutants (Record no. 43618)
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| fixed length control field | 02343nam a2200229Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625140611.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-9368 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | Isolation and characterisation of Gaeumannomyces graminis var. graminis melanin mutants |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | Mycological Research, 103(1), p.99-11, 1999 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | Gaeumannomyces graminis var. graminis produces 1,8-dihydroxynaphthalene (DHN)melanin in its hyphal and hyphopodial cell walls. We isolated G. graminis mutants that were affected in their melanin biosynthesis. One was unable to synthesize DHN-melanin and, because it accumulated 2-hydroxyjuglone, a DHN melanin pathway shunt product, it is most likely to be defective in the reductase that catalyzes the conversion of 1,3,8-trihydroxynaphthalene to vermelone, the penultimate reaction in DHN synthesis. Genetic crosses with our wild-type strain indicated that this trihydroxynaphthalene reductase de®ciency was the result of a single mutation. Another mutant constitutively synthesized DHN melanin and genetic crosses with our wild-type strain suggested that this heavily melanized mutant had a single mutation responsible for its phenotype. This mutant produced more melanin than the wild-type strain as measured by Azure A binding to melanin. The wild type and constitutively melanized mutant hyphae were more hydrophobic and more resistant to lytic enzymes, benomyl, restrictive temperature, and uv light than the non-melanized mutant, which also autolysed more readily. The non-melanized mutant was not more sensitive to heavy metal than the melanized strains. In addition, the non-melanized mutant was unaltered in pathogenicity to rice, whereas the constitutively melanized mutant was ess pathogenic. The constitutively melanized mutant produced less extracellular lytic enzymes than the wild-type and the non-melanized mutant, which may explain its reduced virulence. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Frederick, B.A. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Caesar-Tonthat, T.-C. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Wheeler, M. H. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Sheehan, K. B. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Edens, W. A. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Henson, J. M. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1YqQbdjj0fWuA6qaRSHGYElrDIfs15ds4/view?usp=drivesdk">https://drive.google.com/file/d/1YqQbdjj0fWuA6qaRSHGYElrDIfs15ds4/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
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| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-9368 | 25.06.2025 | 25.06.2025 | Documentos solicitados |