Increased expression of transgene in stably transformed cells of Dunaliella salina by matrix attachment regions (Record no. 43644)

MARC details
000 -LEADER
fixed length control field 02287nam a2200289Ia 4500
003 - CONTROL NUMBER IDENTIFIER
control field MX-MdCICY
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250625140611.0
040 ## - CATALOGING SOURCE
Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-9394
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 250602s9999 xx |||||s2 |||| ||und|d
245 10 - TITLE STATEMENT
Title Increased expression of transgene in stably transformed cells of Dunaliella salina by matrix attachment regions
490 0# - SERIES STATEMENT
Volume/sequential designation Appl Microbiol Biotechnol, 76, p.651-657, 2007
520 3# - SUMMARY, ETC.
Summary, etc. Nuclear matrix attachment regions (MARs)are known to bind specifically to the nuclear scaffold and are thought to influence expression of the transgenes. In our previous studies, a new deoxyribonucleic acid fragment isolated from Dunaliella salina could bind to the nuclear matrix in vitro and had the typical characteristics of MARs. In this study, to investigate effects of MARs on expression of transgenes in the stably transformed cells of D. salina, expression vectors with and without MARs, which contained chloramphenicol acetyltransferase (CAT)reporter gene driven by D. salina ribulose 1,5-bisphosphate carboxylase/ oxygenase promoter, were constructed and delivered, respectively, into cells of D. salina by electroporation. Twenty stably transformed colonies of D. salina were randomly picked out, and CAT gene expression was assayed. The results showed that the CAT enzyme of the colonies of D. salina transformed with the expression vector containing MARs averaged out about 4.5-fold higher than those without MARs, while the transgene expression variation among individuals of transformants decreased threefold. The CAT enzyme in the stably transformed lines was not significantly proportional to the gene copy numbers, suggesting that the effects of MARs on ransgene expression may not be through increasing the transgene copy numbers
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element CHLORAMPHENICOL ACETYLTRANSFERASE
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element DUNALIELLA SALINA
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element MATRIX ATTACHMENT REGION
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element TRANSGENE EXPRESSION
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Wang, T.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Xue, L.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Hou, W.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Yang, B.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Chai, Y.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Ji, X.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Wang, Y.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1SwZ30Mc0CGOKAslAupoOR4VranY9tUDa/view?usp=drivesdk">https://drive.google.com/file/d/1SwZ30Mc0CGOKAslAupoOR4VranY9tUDa/view?usp=drivesdk</a>
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942 ## - ADDED ENTRY ELEMENTS (KOHA)
Source of classification or shelving scheme Clasificación local
Koha item type Documentos solicitados
Holdings
Lost status Source of classification or shelving scheme Damaged status Not for loan Collection Home library Current library Shelving location Date acquired Total checkouts Full call number Date last seen Price effective from Koha item type
  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-9394 25.06.2025 25.06.2025 Documentos solicitados