Expression of Foreign Genes in Dunaliella by Electroporation (Record no. 43648)

MARC details
000 -LEADER
fixed length control field 01526nam a2200289Ia 4500
003 - CONTROL NUMBER IDENTIFIER
control field MX-MdCICY
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250625140612.0
040 ## - CATALOGING SOURCE
Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-9398
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245 10 - TITLE STATEMENT
Title Expression of Foreign Genes in Dunaliella by Electroporation
490 0# - SERIES STATEMENT
Volume/sequential designation Molecular Biotechnology, 30, p.185-192, 2005
520 3# - SUMMARY, ETC.
Summary, etc. An electroporation procedure has been described for introducing plasmid DNA into Dunaliella salina cells. By this procedure, a bulk of plasmid DNA was delivered into the cells and retained for at least 3 d. Reverse transcriptase polymerase chain reaction (RT-PCR)and sequencing analyses indicated that the transcription and pre-mRNA splicing of ble gene (contributing the Zeocin resistance)were detected in the cells as early as 1 h after the electroporation. Individual colonies could retain the resistance to 10 mg/L Zeocin for at least 6 mo. Subsequent Southern blot analysis showed the existence of introduced plasmid DNA inside these colonies. However, most of the cells (approx 90
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element DUNALIELLA SALINA
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element ELECTROPORATION
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element BLE GENE
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element ZEOCIN
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element EPISOMAL DNA
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Sun, Y.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Yang, Z.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Gao, X.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Li, Q.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Zhang, Q.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Xu, Z.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1GZeWNNZwIMb_zvc9WZOwT2XYQEVBznbs/view?usp=drivesdk">https://drive.google.com/file/d/1GZeWNNZwIMb_zvc9WZOwT2XYQEVBznbs/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Source of classification or shelving scheme Clasificación local
Koha item type Documentos solicitados
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Lost status Source of classification or shelving scheme Damaged status Not for loan Collection Home library Current library Shelving location Date acquired Total checkouts Full call number Date last seen Price effective from Koha item type
  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-9398 25.06.2025 25.06.2025 Documentos solicitados