Carotenoid biosynthesis structural genes in carrot (Daucus carota): isolation, sequence-characterization, single nucleotide polymorphism (SNP)markers and genome mapping (Record no. 43725)

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control field MX-MdCICY
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control field 20250625140613.0
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Transcribing agency CICY
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Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-9475
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Title Carotenoid biosynthesis structural genes in carrot (Daucus carota): isolation, sequence-characterization, single nucleotide polymorphism (SNP)markers and genome mapping
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Volume/sequential designation Theoretical and Applied Genetics, 114(4), p.693-704, 2007
520 3# - SUMMARY, ETC.
Summary, etc. Carotenoid pigments are important components of the human diet and carrots are the main dietary sources of the vitamin A precursors a- and bcarotene. Carotenoids play essential biological roles in plants and the genes coding for the carotenoid pathway enzymes are evolutionarily conserved, but little information exists about these genes for carrot. In this study, we utilized published carrot sequences as well as heterologous PCR approaches with primers derived from sequence information of other plant species to isolate 24 putative genes coding for carotenoid biosynthesis enzymes in carrot. Twenty-two of these genes were placed on the carrot genetic linkage map developed from a cross between orange-rooted and whiterooted carrot. The carotenoid genes were distributed in eight of the nine linkage groups in the carrot genome recommending their use for merging maps. Two genes co-localized with a genomic region spanning one of the most significant quantitative trait loci (QTL)for carotenoid accumulation. Carotenoid biosynthesis cDNAs linked to root color mutations and to QTL for carotenoid accumulation may suggest a functional role for them as candidate genes. RACE PCR and reverse transcriptase PCR were used to amplify the full-length transcript for twenty expressed carotenoid biosynthesis genes and sequences were submitted to GenBank. The cloning and sequence information of these genes is useful for PCR-based expression studies and may point toward transgenic approaches to manipulate carotenoid content in carrot.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Just, B.J.
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Personal name Santos, C.A.F.
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Personal name Fonseca, M. E. N.
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Personal name Boiteux, L. S.
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Personal name Oloizia, B. B.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Simon, P. W.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1DKfGujyTnHwYx0AYgqAH4RQ1Ah2fzSGL/view?usp=drivesdk">https://drive.google.com/file/d/1DKfGujyTnHwYx0AYgqAH4RQ1Ah2fzSGL/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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Source of classification or shelving scheme Clasificación local
Koha item type Documentos solicitados
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-9475 25.06.2025 25.06.2025 Documentos solicitados