Use of the 16S-23S ribosomal genes spacer region in studies of prokaryotic diversity (Record no. 43772)

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fixed length control field 02021nam a2200277Ia 4500
003 - CONTROL NUMBER IDENTIFIER
control field MX-MdCICY
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250625140614.0
040 ## - CATALOGING SOURCE
Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-9522
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245 10 - TITLE STATEMENT
Title Use of the 16S-23S ribosomal genes spacer region in studies of prokaryotic diversity
490 0# - SERIES STATEMENT
Volume/sequential designation Journal of MicroBiological Methods, 36(1-2), p.55-64, 1999
520 3# - SUMMARY, ETC.
Summary, etc. The description of microbial diversity by molecular culture-independent techniques most often involves the amplification of the 16S rRNA by PCR gene and either analysis of the diversity of amplified molecules (community fingerprinting)that allows the simultaneous study of many samples or the cloning and sequencing of a significant amount of amplification products. The fact that between the 16S and the 23S genes in the ribosomal operon there is a spacer extremely variable in both sequence and length provides an excellent tool to simplify both approaches. The spacer can be amplified almost as easily as the 16S rDNA taking advantage of conserved nucleotide stretches at the 59 end of the 23S gene and the amplicon can contain different amounts of the 16S rDNA choosing primers at the different conserved areas within this gene. Identified by the acronym RISA (rDNA internal spacer analysis), the spacer addition provides a marker of highly variable size allowing standard separation of the amplification products and the sequence of this hypervariable region is useful in the fine discrimination of operational taxonomic units.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element COMMUNITY FINGERPRINTING
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element RDNA
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element RIBOSOMAL OPERON
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element RISA
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element 16S-23S SPACER
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element ITS
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name García-Martínez,J.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Acinas, S.G.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Antón, A.I.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Rodríguez-Valer, F.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1gFLTqXmeN4qEvaXkDyw2E2xDUWbwFidr/view?usp=drivesdk">https://drive.google.com/file/d/1gFLTqXmeN4qEvaXkDyw2E2xDUWbwFidr/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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Source of classification or shelving scheme Clasificación local
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-9522 25.06.2025 25.06.2025 Documentos solicitados