Phylogenetic and molecular analysis of the ribulose-1,5-bisphosphate carboxylase small subunit gene family in banana (Record no. 43802)

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fixed length control field 02639nam a2200337Ia 4500
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control field MX-MdCICY
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control field 20250625140615.0
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Transcribing agency CICY
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Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-9554
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Title Phylogenetic and molecular analysis of the ribulose-1,5-bisphosphate carboxylase small subunit gene family in banana
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Volume/sequential designation Journal of Experimental Botany, 58(10), p.2685-2697, 2007
520 3# - SUMMARY, ETC.
Summary, etc. Despite being the number one fruit crop in the world, very little is known about the phylogeny and molecular biology of banana (Musa spp.). Six banana rbcS gene families encoding the small subunit of ribulose-1,5- bisphosphate carboxylase/oxygenase from six different Musa spp. are presented. For a comprehensive phylogenetic study using Musa rbcS genes, a total of 57 distinct rbcS sequences was isolated from six accessions that contained different combinations of the A and B ancestral/parental genomes. As a result, five of the six members of the rbcS gene family could be affiliated with the A and/or B Musa genomes and at least three of the six gene families most likely existed before Musa A and B genomes separated. By combining sequence data with quantitative real-time PCR it was determined that the different Musa rbcS gene family members are also often multiply represented in each genome, with the highest copy numbers in the B genome. Expression of some of the rbcS genes varied in intensity and in different tissues indicating differences in regulation. To analyse and compare regulatory sequences of Musa rbcS genes, promoter and terminator regions were cloned for three Musa rbcS genes. Transient transformation assays using promoter- reporter-terminator constructs in maize, wheat, and sugarcane demonstrated that the rbcS-Ma1, rbcSMa3, and rbcS-Ma5 promoters could be useful for transgene expression in heterologous expression systems. Furthermore, the rbcS-Ma1 terminator resulted in a 2-fold increase of transgene expression when directly compared with the widely used Nos terminator
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Topical term or geographic name entry element GENE EXPRESSION
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Topical term or geographic name entry element INTRON
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Topical term or geographic name entry element MUSA
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Topical term or geographic name entry element PHYLOGENOMIC ANALYSIS
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Topical term or geographic name entry element PROMOTER
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Topical term or geographic name entry element RBCS
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Topical term or geographic name entry element RUBISCO
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Topical term or geographic name entry element TERMINATOR
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Personal name Thomas-Hall, S.
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Personal name Campbell, P.R.
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Personal name Carlens, K.
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Personal name Kawanishi, E.
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Personal name Swennen, R.
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Personal name Sagi, L.
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Personal name Schenk, P.M.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1nd2dlR_ybITaB0PLzBMGTpwGEQIpICZP/view?usp=drivesdk">https://drive.google.com/file/d/1nd2dlR_ybITaB0PLzBMGTpwGEQIpICZP/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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Source of classification or shelving scheme Clasificación local
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-9554 25.06.2025 25.06.2025 Documentos solicitados