Phenol-oxidase (laccase)activity in strains of the hyphomycete Chalara paradoxa isolated from olive mill wastewater disposal ponds (Record no. 43838)
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| 000 -LEADER | |
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| fixed length control field | 02114nam a2200241Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625140615.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-9591 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | Phenol-oxidase (laccase)activity in strains of the hyphomycete Chalara paradoxa isolated from olive mill wastewater disposal ponds |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | Enzyme and Microbial Technology, 26(7), p.484-490, 2000 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | Production of laccase activity by nine strains of Chalara paradoxa isolated from olive mill wastewater disposal ponds were studied. Enzyme extracts obtained from cultured broths by adsorption on hydroxyapatite showed a single band of laccase activity on ABTS after polyacrylamide gel electrophoresis (PAGE). They showed small mobility differences, with molecular masses of 67 to 68 kDa. Enzymes from the different strains oxidized a variety of phenolic and non-phenolic substances, and they could be divided into two groups according to their relative activities on substrates. All laccases showed a dual pH dependence of activity, with a maximum in the range of pH 3.0 to 4.5 for ABTS, o-dianisidine and 2,6-dimethoxyphenol, and pH 6.0 (Group 1)or pH 6.5 (Group 2)for syringaldazine and other substrates. Optimal temperatures were in the range of 10 to 28°C for two strains (maximum at 10°C)and 10 to 37°C for the rest. The different enzymes were partially inactivated by heating at 60°C nd totally inactivated at 70°C. Laccases were stable in a pH range of 3.0 to 9.0 (except for strain 36A, which was partially inactivated at pH 3.0), but became inactivated at pH 2.0. Altogether these data suggest that Ch. paradoxa strains produce different laccase isoenzymes. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | PHENOL-OXIDASE |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | LACCASE |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | CHALARA PARADOXA |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Robles, A. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Lucas, R. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Alvarez De Cienfuegos, G. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Gálvez, A. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1OetCkAKH7RuTKTjfltyCj_yC-KneE4EA/view?usp=drivesdk">https://drive.google.com/file/d/1OetCkAKH7RuTKTjfltyCj_yC-KneE4EA/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-9591 | 25.06.2025 | 25.06.2025 | Documentos solicitados |