A one-step method to convert vectors into binary vectors suited for Agrobacterium -mediated transformation (Record no. 43840)

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control field MX-MdCICY
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control field 20250625140615.0
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Transcribing agency CICY
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Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-9593
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245 10 - TITLE STATEMENT
Title A one-step method to convert vectors into binary vectors suited for Agrobacterium -mediated transformation
490 0# - SERIES STATEMENT
Volume/sequential designation Current Genetics, 45(4), p.242-248, 2004
520 3# - SUMMARY, ETC.
Summary, etc. Bacterial artificial chromosomes (BACs)are widely used for the construction of physical maps, positional-cloning and whole-genome sequencing strategies. Unfortunately, their use for functional genomics is limited, as currently there is no efficient method to use BACs directly for complementation. We describe a novel strategy for one-step conversion of any BAC into a binary BAC (BIBAC). Using Agrobacterium umefaciens, these BIBACs can be efficiently transformed to virtually all organisms, including plants, fungi, yeasts and human cells. As the strategy is based on in vivo recombineering and does not depend on restriction sites, it is applicable to any vector. To show the feasibility of the method five BACs, containing 0-75 kb of fungal DNA, were converted into BIBACs. These were subsequently transformed to the plant pathogenic fungus Fusarium oxysporum f.sp. lycopersici and to Aspergillus awamori, a filamentous fungus often used for large-scale protein production. Molecular characterisation of the transformants showed that the BIBACs were efficiently transferred to the fungi and stably integrated into their genomes.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element BINARY VECTOR
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element BACTERIAL ARTIFICIAL
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element CHROMOSOME
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element BIBAC
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element RECOMBINEERING
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Takken, F.L.W.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Van Wijk, R.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Michielse, C.B.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Houterman, P.M.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Ram, A.F.J.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Cornelissen, B.J.C.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1irTNIcA51dUsJr-duRe3sdqsC5IgWEjj/view?usp=drivesdk">https://drive.google.com/file/d/1irTNIcA51dUsJr-duRe3sdqsC5IgWEjj/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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Source of classification or shelving scheme Clasificación local
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-9593 25.06.2025 25.06.2025 Documentos solicitados