A new method for screening and isolation of hypersecretion mutants in Aspergillus niger (Record no. 43844)
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| fixed length control field | 02114nam a2200205Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625140615.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-9597 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | A new method for screening and isolation of hypersecretion mutants in Aspergillus niger |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | Appl Microbiol Biotechnol, 69(6), p.711-717, 2006 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | Although filamentous fungi have a unique property of secreting a large amount of homologous extracellular proteins, the use of filamentous fungi as hosts for the production of heterologous proteins is limited because of the low production levels that are generally reached. Here, we report a general screening method for the isolation of mutants with increased protein production levels. The screening method makes use of an Aspergillus niger strain that lacks the two major amylolytic enzymes, glucoamylase (GlaA)and acid amylase (AamA). The double-mutant strain grows poorly on starch and its growth is restored after reintroducing the catalytic part of the glucoamylase gene (GlaA512). We show that the fusion of a heterologous protein, a laccase from Pleurotus ostreatus (Pox2), to the catalytic part of glucoamylase (GlaA512-Pox2)severely hampers efficient production of the glucoamylase protein, resulting in a slow-growth phenotype on starch. Laccasehypersecreting mutants were obtained by isolating mutants that displayed improved growth on starch plates. The mutant with the highest growth rate on starch displayed the highest laccase activity, indicating that increased glucoamylase protein levels are correlated with higher laccase production levels. In principle, our method can be applied to any low-produced heterologous protein that is secreted as a fusion with the glucoamylase protein. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Weenink, X.O. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Punt, P.J. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Van Den Hondel, C.A.M.J.J. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Ram, A.F.J. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/11-5AO2mWfhKjuaZPDsVmxqkqLmHsWSY4/view?usp=drivesdk">https://drive.google.com/file/d/11-5AO2mWfhKjuaZPDsVmxqkqLmHsWSY4/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
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| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-9597 | 25.06.2025 | 25.06.2025 | Documentos solicitados |