Genetic or chemical protease inhibition causes significant changes in the Bacillus subtilis exoproteome (Record no. 43875)
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| fixed length control field | 02424nam a2200325Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625140616.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-9628 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | Genetic or chemical protease inhibition causes significant changes in the Bacillus subtilis exoproteome |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | Proteomics, 8(13), p.2704-2713, 2008 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | Bacillus subtilis is a prolific producer of enzymes and biopharmaceuticals. However, the susceptibility of heterologous proteins to degradation by (extracellular)proteases is a major limitation for use of B. subtilis as a protein cell factory. An increase in protein production levels has previously been achieved by using either protease-deficient strains or addition of protease inhibitors to B. subtilis cultures. Notably, the effects of genetic and chemical inhibition of proteases have thus far not been compared in a systematic way. In the present studies, we therefore compared the exoproteomes of cells in which extracellular proteases were genetically or chemically inactivated. The results show substantial differences in the relative abundance of various extracellular proteins. Furthermore, a comparison of the effects of genetic and/or chemical protease inhibition on the stress response triggered by (over)production of secreted proteins showed that chemical protease inhibition provoked a genuine secretion stress response. From a physiological point of view, this suggests that the deletion of protease genes is a better way to prevent product degradation than the use of protease inhibitors. Importantly however, studies with human interleukin-3 show that chemical protease inhibition can result in improved production of protease-sensitive secreted proteins even in mutant strains lacking eight extracellular proteases. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | ALPHA-AMYLASE |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | EXOPROTEOME |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | INTERLEUKIN-3 |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | PROTEASE INHIBITION |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | SECRETOME |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Westers, L. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Westers, H. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Zanen, G. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Antelmann, H. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Hecker, M. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Noone, D. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Devine, K.M. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Devine, K.M. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Quax, W.J. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1TaOfdM9Y2AFXqLHF58jEkCZbZS2hdkFn/view?usp=drivesdk">https://drive.google.com/file/d/1TaOfdM9Y2AFXqLHF58jEkCZbZS2hdkFn/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
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| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-9628 | 25.06.2025 | 25.06.2025 | Documentos solicitados |