A Simple Silica-based Method for Metagenomic DNA Extraction from Soil and Sediments (Record no. 43895)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 01437nam a2200265Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625140616.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-9648 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | A Simple Silica-based Method for Metagenomic DNA Extraction from Soil and Sediments |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | Molecular Biotechnology, 40(1), p.13-17, 2008 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | A new method is described for extraction of metagenomic DNA from soil and sediments which is based on DNA adsorption to silica without the use of phenol, ethanol precipitation or a cesium chloride gradient. Highquality DNA was obtained, and PCR inhibition was overcome by adding bovine serum albumin and adjusting magnesium concentration. By using PCR-DGGE with Firmicutes and lactic acid bacteria-specific primers the extracted metagenomic DNA was shown to contain a mixture of bacterial genomes. This method can be used for screening bacterial diversity in soil and sediment samples |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | METAGENOMIC DNA |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | MICROBIAL COMMUNITY |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | SEDIMENTS |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | SOILS |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | DGGE |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Rojas-Herrera, R. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Narváez-Zapata, J. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Zamudio-Maya, M. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Mena-Martínez, M.E. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1uGN7ygfoHLFfEf6Vcm_zY69fIX3Tt5Ex/view?usp=drivesdk">https://drive.google.com/file/d/1uGN7ygfoHLFfEf6Vcm_zY69fIX3Tt5Ex/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-9648 | 25.06.2025 | 25.06.2025 | Documentos solicitados |