Isolation and Purification of RNA from Tissues Rich in Polyphenols, Polysaccharides, and Pigments of Annatto (Bixa orellana L.) (Record no. 44014)

MARC details
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fixed length control field 02082nam a2200277Ia 4500
003 - CONTROL NUMBER IDENTIFIER
control field MX-MdCICY
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250625140619.0
040 ## - CATALOGING SOURCE
Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-9768
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245 10 - TITLE STATEMENT
Title Isolation and Purification of RNA from Tissues Rich in Polyphenols, Polysaccharides, and Pigments of Annatto (Bixa orellana L.)
490 0# - SERIES STATEMENT
Volume/sequential designation Mol. Biotechnol., 37, p.220-224, 2007
520 3# - SUMMARY, ETC.
Summary, etc. The tropical plant Bixa orellana L. (annatto)produces an array of natural products, including the pigment bixin used in the food and cosmetics industries. In order to understand the biochemical and molecular basis of the biosynthesis of these natural products, a reliable method for isolating high yields of high-quality RNA is required. Here we described a successful and reproducible method for isolation and purification of high-quantity and high-quality RNA from different tissues of annatto. This protocol overcomes the usual problems associated with large amounts of polyphenols, polysaccharides, pigments, and other secondary metabolites that are not easily removed by conventional extraction procedures. Furthermore, the proposed protocol can be easily carried out in any laboratory and it could also be extended to isolate RNA from other plant species showing similar abundance of compounds that interfere with RNA extractions. The yield and quality of the RNA were monitored by spectrophotometric analysis, separation on agarose gel, Reverse Transcription- Polymerase Chain Reaction (RT-PCR), and construction of a cDNA library.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element RNA EXTRACTION
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element MATAB (MIXED ALKYL TRIMETHYL AMMONIUM BROMIDE)
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element RT-PCR
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element CDNA LIBRARY
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Rodrigues, S.M.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Soares, V.L.F.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name De Oliveira, T.M.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Gesteira, A.S.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Otoni, W.C.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Costa, M.C.G.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/18yrJxSOYp8uft5VTLDSDjXTvqI7zPKcE/view?usp=drivesdk">https://drive.google.com/file/d/18yrJxSOYp8uft5VTLDSDjXTvqI7zPKcE/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Source of classification or shelving scheme Clasificación local
Koha item type Documentos solicitados
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-9768 25.06.2025 25.06.2025 Documentos solicitados