Expression of a Foreign Gene in Chlamydomonas reinhardtii Chloroplast (Record no. 44039)

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fixed length control field 01640nam a2200289Ia 4500
003 - CONTROL NUMBER IDENTIFIER
control field MX-MdCICY
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250625140619.0
040 ## - CATALOGING SOURCE
Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-9793
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245 10 - TITLE STATEMENT
Title Expression of a Foreign Gene in Chlamydomonas reinhardtii Chloroplast
490 0# - SERIES STATEMENT
Volume/sequential designation Journal of BioScience and BioEngineering, 87(3), p.307-314, 1999
520 3# - SUMMARY, ETC.
Summary, etc. Chimeric genes for expression of a foreign gene in the Chlamydomonas reinhardtii chloroplast were constructed. These chimeric genes are composed of the promoter from chloroplast genes, rbcL, psbA, and atpA, S- and S/-untranslated regions, and the Escherichia coli ,9-glucuronidase (GUS)structural gene (uidA)as a foreign gene. Three types of chloroplast transformants (RG, PG, and AG), which contained the rbcL-uidA, psbA-uidA, and atpA-uidA chimerlc genes integrated in the chloroplast genome, were generated by particle bombardment. The AG transformant grown under photoautotrophlc conditions showed the highest GUS activity (130 nmol/min/mg protein)so far reported in C. reinhurdtii, and the accumulated GUS protein accounted for 0.08
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element CHLAMYDOMONAS REINHARDTII
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element CHLOROPLAST
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Topical term or geographic name entry element TRANSFORMATION
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element PROMOTER
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Topical term or geographic name entry element EXPRESSION
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element P-GLUCURONIDASE
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Ishikura, K.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Takaoka, Y.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Kato, K.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Sekine, M.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Yoshida, K.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/132OEbRdj6o33yG9wFEFXjOmbC5kSJtIs/view?usp=drivesdk">https://drive.google.com/file/d/132OEbRdj6o33yG9wFEFXjOmbC5kSJtIs/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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Source of classification or shelving scheme Clasificación local
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-9793 25.06.2025 25.06.2025 Documentos solicitados