Regulatory interaction of the Ga protein with phospholipase A2 in the plasma membrane of Eschscholzia californica (Record no. 44079)
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| fixed length control field | 02391nam a2200277Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625140620.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-9833 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | Regulatory interaction of the Ga protein with phospholipase A2 in the plasma membrane of Eschscholzia californica |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | The Plant Journal, 52(6), p.1041-1051, 2007 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | Plant heterotrimeric G-proteins are involved in a variety of signaling pathways, though only one a and a few bc isoforms of their subunits exist. In isolated plasma membranes of California poppy (Eschscholzia californica), the plant-specific Ga subunit was isolated and identified immunologically and by homology of the cloned gene with that of several plants. In the same membrane, phospholipase A2 (PLA2)was activated by yeast elicitor only if GTPcS (an activator of Ga)was present. From the cholate-solubilized membrane proteins, PLA2 was co-precipitated together with Ga by a polyclonal antiserum raised against the recombinant Ga. In this immunoprecipitate and in the plasma membrane (but not in the Ga-free supernatant)PLA2 was stimulated by GTPcS. Plasma membranes and immunoprecipitates obtained from antisense transformants with a low Ga content allowed no such stimulation. An antiserum raised against the C-terminus (which in animal Gas is located near the target coupling site)precipitated Ga without any PLA2 activity. Using non-denaturing PAGE, complexes of solubilized plasma membrane proteins were visualized that contained Ga plus PLA2 activity and dissociated at pH 9.5. At this pH, PLA2 was no longer stimulated by GTPcS. It is concluded that a distinct fraction of the plasma membrane-bound PLA2 exists in a detergent-resistant complex with Ga that can be dissociated at pH 9.5. This complex allows the Ga-mediated activation of PLA2. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | PLANT G(ALPHA)PROTEIN |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | PHOSPHOLIPASE A2 |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | PLANT SIGNAL TRANSFER |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | MEMBRANE PROTEIN COMPLEXES |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | PLASMA MEMBRANE PROTEINS |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Heinze, M. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Steighardt, J. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Gesell, A. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Schwartze, W. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Roos, W. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1mcsH5kES-DcIjOmuFr6xALrTR1Rwpg44/view?usp=drivesdk">https://drive.google.com/file/d/1mcsH5kES-DcIjOmuFr6xALrTR1Rwpg44/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
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| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-9833 | 25.06.2025 | 25.06.2025 | Documentos solicitados |