Differential protein expression in Colletotrichum acutatum : changes associated with reactive oxygen species and nitrogen starvation implicated in pathogenicity on strawberry (Record no. 44179)
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| fixed length control field | 02862nam a2200241Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625140622.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-9936 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | Differential protein expression in Colletotrichum acutatum : changes associated with reactive oxygen species and nitrogen starvation implicated in pathogenicity on strawberry |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | Molecular Plant Pathology, 9(2), p.171-190, 2008 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | The cellular outcome of changes in nitrogen availability in the context of development and early stages of pathogenicity was studied by quantitative analysis of two-dimensional gel electrophoresis of Colletotrichum acutatum infecting strawberry. Significant alterations occurred in the abundance of proteins synthesized during appressorium formation under nitrogen-limiting conditions compared with a complete nutrient supply. Proteins that were up- or down-regulated were involved in energy metabolism, nitrogen and amino acid metabolism, protein synthesis and degradation, response to stress and reactive oxygen scavenging. Members belonging to the reactive oxygen species (ROS)scavenger machinery, superoxide dismutase and glutathione peroxidase, were up-regulated at the appressorium formation stage, as well as under nitrogen-limiting conditions relative to growth with a complete nutrient supply, whereas abundance of bifunctional catalase was up-regulated predominantly at the appressorium formation stage. Fungal ROS were detected within germinating conidia during host pre-penetration, penetration and colonization stages, accompanied by plant ROS, which were abundant in the apoplastic space. Application of exogenous antioxidants quenched ROS production and reduced the frequency of appressorium formation. Up-regulation in metabolic activity was detected during appressorium formation and nutrient deficiency compared with growth under complete nutrient supply. Enhanced levels of proteins related to the glyoxylate cycle and lipid metabolism (malate dehydrogenase, formate dehydrogenase and acetyl-CoA acetyltransferase)were observed at the appressorium formation stage, in contrast to down-regulation of isocitrate dehydrogenase. The present study demonstrates that appressoria formation processes, occurring under nutritional deprivation, are accompanied by metabolic shifts, and that ROS production is an early fungal response that may modulate initial stages of pathogen development. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Brown, S.H. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Yarden, O. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Gollop, N. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Chen, S. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Zveibil, A. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Belausov, E. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Freeman, S. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1FIJoiGXH5RQkCT_T0VMzgIuWxPPJAN-X/view?usp=drivesdk">https://drive.google.com/file/d/1FIJoiGXH5RQkCT_T0VMzgIuWxPPJAN-X/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
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| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-9936 | 25.06.2025 | 25.06.2025 | Documentos solicitados |