The Arabidopsis thaliana genome has multiple divergent forms of phosphoinositol-specific phospholipase C1 (Record no. 44197)

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control field MX-MdCICY
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control field 20250625140622.0
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Transcribing agency CICY
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Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-9954
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Title The Arabidopsis thaliana genome has multiple divergent forms of phosphoinositol-specific phospholipase C1
490 0# - SERIES STATEMENT
Volume/sequential designation Gene, 202(1-2), p.151-156, 1997
520 3# - SUMMARY, ETC.
Summary, etc. Highly degenerate primers to conserved regions of the eukaryotic phosphoinositol-specific phospholipase C (PLC)were used to amplify fragments of plant PLCs from Arabidopsis thaliana genomic DNA. Eight completely different fragment sequences that showed high homology to PLCs of both animals and plants were isolated. The variation between these putative PLCs was high and suggests that, like animals, plants have multiple isoforms of PLC. Using one of the PCR clones, we isolated a corresponding full-length Arabidopsis PLC gene (ATHATPLC1G), and sequence analysis indicated that it was most like a delta-type PLC. This gene is 2.5 kb and contains seven introns, all but one of which has intron/exon border sequences that conform to the Arabidopsis consensus. The structural complexity of the gene is relatively simple compared to mammalian b-type PLCs that can be 15 kb long with up to 30 introns. The plant gene is a single copy and was mapped to four Arabidopsis YACs, one located on chromosome 2. The promoter region contained two TATA-like elements at -43 and -185 and other putative regulatory elements that suggest that this PLC is hormonally regulated. This is the first plant PLC gene and the first delta type-PLC gene from a higher organism to be sequenced.
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Topical term or geographic name entry element SIGNAL TRANSDUCTION
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element PHOSPHOINOSITOL-SPECIFIC PHOSPHOLIPASE C
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element DEGENERATE PCR AMPLIFICATION
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element PLANT GENE REGULATION
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Hartweck, L.M.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Llewellyn, D.J.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Dennis, E.S.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/115mHeKrLDsw4GHOsZCKQq43W9sNE3Nbg/view?usp=drivesdk">https://drive.google.com/file/d/115mHeKrLDsw4GHOsZCKQq43W9sNE3Nbg/view?usp=drivesdk</a>
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Source of classification or shelving scheme Clasificación local
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-9954 25.06.2025 25.06.2025 Documentos solicitados