Efficient microRNA capture and bar-coding via enzymatic oligonucleotide adenylation (Record no. 44253)

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control field MX-MdCICY
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control field 20250625140623.0
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Transcribing agency CICY
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Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-10011
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Title Efficient microRNA capture and bar-coding via enzymatic oligonucleotide adenylation
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Volume/sequential designation Nature Methods, 5(9), p.777-779, 2008
520 3# - SUMMARY, ETC.
Summary, etc. Here we report a highly efficient and simplified strategy to preadenylate bar-coded oligonucleotides designed for microRNA (miRNA)capture and multiplex analysis. Using this approach, we enzymatically preadenylated bar-coded oligonucleotides with high efficiency when compared to the chemical method currently used by miRNA investigators. As a case study, we used these oligonucleotides in an ATP-independent ligation to miRNAs, suggesting the utility of our method in end-capture protocols and high-throughput sequencing applications.
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Personal name Vigneault, F.
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Personal name Michael Sismour, A.
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Personal name Church, G.M.
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Uniform Resource Identifier <a href="https://drive.google.com/file/d/14KG349UEHYc5WFS7l2_wCD6l6WwoAMZv/view?usp=drivesdk">https://drive.google.com/file/d/14KG349UEHYc5WFS7l2_wCD6l6WwoAMZv/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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Source of classification or shelving scheme Clasificación local
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-10011 25.06.2025 25.06.2025 Documentos solicitados