Global identification of microRNA-target RNA pairs by parallel analysis of RNA ends (Record no. 44254)

MARC details
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fixed length control field 02113nam a2200337Ia 4500
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control field MX-MdCICY
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control field 20250625140623.0
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Transcribing agency CICY
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Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-10012
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Title Global identification of microRNA-target RNA pairs by parallel analysis of RNA ends
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Volume/sequential designation Nature Biotechnology, 26(8), p.941-946, 2008
520 3# - SUMMARY, ETC.
Summary, etc. MicroRNAs (miRNAs)are important regulatory molecules in most eukaryotes and identification of their target mRNAs is essential for their functional analysis. Whereas conventional methods rely on computational prediction and subsequent experimental validation of target RNAs, we directly sequenced >28,000,000 signatures from the 5_O ends of polyadenylated products of miRNA-mediated mRNA decay, isolated from inflorescence tissue of Arabidopsis thaliana, to discover novel miRNA.target RNA pairs. Within the set of ~27,000 transcripts included in the 8,000,000 nonredundant signatures, several previously predicted but nonvalidated targets of miRNAs were found. Like validated targets, most showed a single abundant signature at the miRNA cleavage site, particularly in libraries from a mutant deficient in the 5_O-to-3_O exonuclease AtXRN4. Although miRNAs in Arabidopsis have been extensively investigated, working in reverse from the cleaved targets resulted in the identification and validation of novel miRNAs. This versatile approach will affect the study of other aspects of RNA processing beyond miRNA.target RNA pairs.
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Personal name German, M.A.
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Personal name Pillay, M.
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Personal name Jeong, D.
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Personal name Hetawal, A.
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Personal name Luo, S.
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Personal name Janardhanan, P.
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Personal name Kannan, V.
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Personal name Kannan, V.
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Personal name Nobuta, K.
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Personal name German, R.
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Personal name De Paoli, E.
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Personal name Lu, C.
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Personal name Schroth, G.
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Personal name Meyers, B.C.
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Personal name Green, P.J.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1mihwvVvEdoO24eASeuTePwT-IDihPHWk/view?usp=drivesdk">https://drive.google.com/file/d/1mihwvVvEdoO24eASeuTePwT-IDihPHWk/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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Source of classification or shelving scheme Clasificación local
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-10012 25.06.2025 25.06.2025 Documentos solicitados