Detection of Plant Genes Using a Rapid, Nonorganic DNA Purification Method (Record no. 44289)
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| 000 -LEADER | |
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| fixed length control field | 02046nam a2200217Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625140624.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-10047 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | Detection of Plant Genes Using a Rapid, Nonorganic DNA Purification Method |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | BioTechniques, 28(2), p.346-350, 2000 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | We have developed a simple procedure for the preparation of plant genomic DNA using FTA paper. Plant leaves were crushed against FTA paper, and the genomic DNA was purified using simple, nonorganic reagents. The 18S rRNA gene and the gene encoding the ribulose-1, 5-bisphosphate carboxylase/oxygenase large subunit (rbcL)from the chloroplast genome were detected by PCR amplification of DNA on FTA paper. DNA amplification was successful using extracts from 16 dicot and monocot plants. Studies of specific plant extracts revealed that extracts of leaf samples could be collected and stored at room temperature on FTA paper without a decrease in the DNA amplification success rate for more than a month. Both the 18S RNA gene and the rbcL gene were detected in the genomic DNA isolated from various soybean cultivars stored in this manner. Furthermore, by modestly increasing the number of cycles of DNA amplification, we were able to detect the uidA gene in transgenic tobacco and rice leaves as well as a single copy gene linked to the resistance gene of cyst nematode race 3 using genomic DNA isolated on FTA paper. These results demonstrate that genomic DNA isolated using FTA paper can be used for the detection of plant genes, from a wide range of plants with either high or low gene copy number and of either nuclear or cytoplasmic origin. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Lin, J.J. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Fleming, R. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Kuo, J. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Matthews, B.F. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Saunders, J.A. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1n7gW_fpQHmYrye93blaZAX4XzIynDFeP/view?usp=drivesdk">https://drive.google.com/file/d/1n7gW_fpQHmYrye93blaZAX4XzIynDFeP/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
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| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-10047 | 25.06.2025 | 25.06.2025 | Documentos solicitados |