Characterization of Arabidopsis thaliana mismatch specific endonucleases: application to mutation discovery by TILLING in pea (Record no. 44341)
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| fixed length control field | 02511nam a2200325Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625140625.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-10102 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | Characterization of Arabidopsis thaliana mismatch specific endonucleases: application to mutation discovery by TILLING in pea |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | The Plant Journal, 51(6), p.1116-1125, 2007 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | Scanning DNA sequences for mutations and polymorphisms has become one of the most challenging, often expensive and time-consuming obstacles in many molecular genetic applications, including reverse genetic and clinical diagnostic applications. Enzymatic mutation detection methods are based on the cleavage of heteroduplex DNA at the mismatch sites. These methods are often limited by the availability of a mismatchspecific endonuclease, their sensitivity in detecting one allele in a pool of DNA and their costs. Here, we present detailed biochemical analysis of five Arabidopsis putative mismatch-specific endonucleases. One of them, ENDO1, is presented as the first endonuclease that recognizes and cleaves all types of mismatches with high efficiency.Wereport on a very simple protocol for the expression and purification of ENDO1. The ENDO1system could be exploited in a wide range of mutation diagnostic tools. In particular, we report the use of ENDO1 for discovery of point mutations in the gibberellin 3b-hydrolase gene of Pisum sativum. Twenty-one independent mutants were isolated, five of these were characterized and two new mutations affecting internodes length were identified. To further evaluate the quality of the mutant population we screened for mutations in four other genes and identified 5-21 new alleles per target. Based on the frequency of the obtained alleles we concluded that the pea population described here would be suitable for use in a large reverse-genetics project |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | MUTATION |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | HETERODUPLEX DNA |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | ENDONUCLEASE |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | TILLING |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Triques, K. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Sturbois, B. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Gallais, S. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Dalmais, M. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Chauvin, S. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Clepet, C. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Aubourg, S. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Aubourg, S. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Caboche, M. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Bendahmane, A. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1lick-7fHX3FKjU1iaUvE0AYtZd9yciXB/view?usp=drivesdk">https://drive.google.com/file/d/1lick-7fHX3FKjU1iaUvE0AYtZd9yciXB/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
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| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-10102 | 25.06.2025 | 25.06.2025 | Documentos solicitados |