The yeast vacuolar membrane proteome (Record no. 44441)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 02667nam a2200289Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625140627.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-10202 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | The yeast vacuolar membrane proteome |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | Molecular & Cellular Proteomics, 8, p.380-392, 2009 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | Transport of solutes between the cytosol and the vacuolar lumen is of crucial importance for various functions of vacuoles, including ion homeostasis, detoxification, storage of different molecules such as amino acids, phosphate and calcium ions, and proteolysis. In order to identify proteins that catalyze solute transport across the vacuolar membrane, the membrane proteome of purified Saccharomyces cerevisiae vacuoles was analyzed. Subtractive proteomics was used to distinguish contaminants from true vacuolar proteins by comparing the relative abundances of proteins in pure and crude preparations. A robust statistical analysis combining Enrichment Ranking with the double-boundary iterative Group Analysis revealed that 148 proteins were significantly enriched in the pure vacuolar preparations. Among these proteins were well-characterized vacuolar proteins, such as the subunits of the V-ATPase, but also proteins that had not previously been assigned to a cellular location, many of which are likely novel vacuolar membrane transporters e.g. for nucleosides and oligopeptides. Although the majority of contaminating proteins from other organelles were depleted from the pure vacuolar membranes, some proteins annotated to reside in other cellular locations were enriched along with the vacuolar proteins. In many cases the enrichment of these proteins is biologically relevant, and we discuss that a large group is involved in membrane fusion and protein trafficking to vacuoles and may have multiple localizations. Other proteins are degraded in vacuoles and in some cases database annotations are likely to be incomplete or incorrect. Our work provides a wealth of information on vacuolar biology and a solid basis for further characterization of vacuolar functions. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | ORGANELLE PROTEOMICS |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | VACUOLE |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | MEMBRANE PROTEINS |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | ITERATIVE GROUP ANALYSIS |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | MEMBRANE TRANSPORT |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Wiederhold, E. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Gandhi, T. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Permentier, H.P. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Breitling, R. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Poolman, B. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Slotboom, D.J. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1-surI__nYNQsCka9kz8voTU34chYjn8o/view?usp=drivesdk">https://drive.google.com/file/d/1-surI__nYNQsCka9kz8voTU34chYjn8o/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
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| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-10202 | 25.06.2025 | 25.06.2025 | Documentos solicitados |