Perchloric Acid Enhances Sensitivity and Reproducibility of the Ferric-Xylenol Orange Peroxide Assay (Record no. 44448)
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| fixed length control field | 01783nam a2200241Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625140627.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-10209 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | Perchloric Acid Enhances Sensitivity and Reproducibility of the Ferric-Xylenol Orange Peroxide Assay |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | Analytical BioChemistry, 304(1), p.42-46, 2002 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | The ferrous oxidation in xylenol orange (FOX)assay for hydroperoxides suffers from very narrow pH optimum in the range 1.7-1.8. Most published protocols recommend 25 mM sulfuric acid as the solvent, but this in practice does not ensure the maintenance of correct pH in the presence of materials such as samples of biological origin. Substitution of perchloric for the sulfuric acid resulted in a lowering of the optimum pH of the assay to 1.1, a decreased dependence of the absorbance of the ferric-xylenol orange complex on acid concentration and decreased sensitivity to added compounds. Molar absorption coefficients of hydrogen peroxide, cumene, and butyl hydroperoxides and of hydroperoxide groups generated in oxidized protein and lipids were determined and found to be higher than in sulfuric acid. The optimum concentration of perchloric acid proved to be 110 mM. The new assay was designated as PCA-FOX, to distinguish it from the FOX methods based on sulfuric acid. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | HYDROPEROXIDE |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | LIPID PEROXIDE |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | PROTEIN PEROXIDE |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | XYLENOL ORANGE |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | PERCHLORIC ACID |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Gay, C.A. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Gebicki, J.M. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1VZ-umKfa9z0Hc2qcXOjC-MY2v80vlQp9/view?usp=drivesdk">https://drive.google.com/file/d/1VZ-umKfa9z0Hc2qcXOjC-MY2v80vlQp9/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
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| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-10209 | 25.06.2025 | 25.06.2025 | Documentos solicitados |