Development and Selection for Homozygous Transgenic Papaya Seedling (Record no. 44490)

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fixed length control field 02231nam a2200265Ia 4500
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control field MX-MdCICY
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control field 20250625140628.0
040 ## - CATALOGING SOURCE
Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-10251
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245 10 - TITLE STATEMENT
Title Development and Selection for Homozygous Transgenic Papaya Seedling
490 0# - SERIES STATEMENT
Volume/sequential designation Acta Horticulturae, 740, p.177-182, 2007
520 3# - SUMMARY, ETC.
Summary, etc. Somatic embryos of papaya lines were genetically engineered via Agrobacterium tumefaciens to contain the coat protein gene of the Virgin Islands strain of Papaya Ringspot Virus (PRSV). Regenerated plants, R0, had resistance to PRSV. Plants from the R1 generation segregated for resistance. Self-pollinations were conducted to develop inbred homozygous PRSV-resistant papaya lines. A quick efficient method for the screening and selection of transgenic papaya seedlings was needed to identify homozygous transgenic papaya lines. Transgenic lines of papaya also contained the neomycin phosphotransferase gene which was used for in vitro selection of transgenic papaya embryos. Papaya R2 seedlings were sprayed twice at 10 and 17 days with kanamycin in combination with dimethyl sulfoxide (DMSO). Non-transgenic papaya seedlings developed yellow spots within four days. DMSO enhanced the effect of kanamycin-susceptible plants, but, did not itself induce the formation of yellow spots in the transgenic lines. Following the success of the initial trial, seedlings of eleven transgenic R3 papaya lines were screened using 1,000 mg/L kanamycin with 1 ml/L DMSO. Three papaya lines produced no yellowing following two applications of kanamycin and DMSO, indicating homozygous lines for resistance. Through the use of kanamycin and DMSO, homozygous transgenic papaya lines can be successfully identified in the greenhouse at the seedling stage.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element CARICA
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element KANAMYCIN
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element DMSO
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element CHLOROSIS
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element NPTII
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Zimmerman, T.W.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Joseph, L.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name St. Brice, N.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Kowalski, J.A.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/11zYs6YHmvm7megRNBWhQ_rl6pVbA6gJR/view?usp=drivesdk">https://drive.google.com/file/d/11zYs6YHmvm7megRNBWhQ_rl6pVbA6gJR/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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Source of classification or shelving scheme Clasificación local
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-10251 25.06.2025 25.06.2025 Documentos solicitados