MARC details
| 000 -LEADER |
| fixed length control field |
02180nam a2200265Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
| control field |
MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
| control field |
20250625140628.0 |
| 040 ## - CATALOGING SOURCE |
| Transcribing agency |
CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
B-10278 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
| fixed length control field |
250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT |
| Title |
Isolation and transcript analysis of two-component histidine kinase gene Le.nik1 in Shiitake mushroom, Lentinula edodes |
| 490 0# - SERIES STATEMENT |
| Volume/sequential designation |
Mycological Research, 112(1), p.108-116, 2008 |
| 520 3# - SUMMARY, ETC. |
| Summary, etc. |
Le.nik1, a two-component histidine kinase gene of Lentinula edodes, the Shiitake mushroom, was identified. The relationship between this two-component signal transduction system and mushroom development was studied. We used a modified RNA arbitrarily-primed PCR (RAP-PCR)method to isolate Le.nik1 as a differentially expressed gene during L. edodes development. We determined the 6.29 kb full-length cDNA sequence of Le.nik1. It had high sequence homology to Neurospora crassa nik1, which encoded a histidine kinase essential for development and osmotic response. In L. edodes, the expression level of Le.nik1 was highest during primordium formation and fruiting body maturation. The transcripts were localized predominantly in the developing hymenophores, or mushroom gills, which may indicate the role of a two-component signal transduction systemin cell differentiation during mushroom development. Mannitol stress influenced transcript expression of Le.nik1, suggesting that it may be involved in osmo-sensing and regulation. To our knowledge, this is the first report on the two-component system in mushrooms and the first analysis on the distribution of Le.nik1 transcript in the course of fruiting body formation and in parts of fruiting bodies. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
| Topical term or geographic name entry element |
IN SITU RNA-RNA HYBRIDIZATION |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
| Topical term or geographic name entry element |
MANNITOL STRESS |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
| Topical term or geographic name entry element |
OSMOREGULATION |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
| Topical term or geographic name entry element |
TRANSCRIPT EXPRESSION AND LOCALIZATION |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
| Topical term or geographic name entry element |
TWO-COMPONENT SYSTEM |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
| Personal name |
Szeto, C.Y.Y. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
| Personal name |
Wong, Q.W.L. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
| Personal name |
Leung, G.S. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
| Personal name |
Kwan, H.S. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS |
| Uniform Resource Identifier |
<a href="https://drive.google.com/file/d/10uJkfG51l1BV9jq1gPlzPz96kGqZaFIX/view?usp=drivesdk">https://drive.google.com/file/d/10uJkfG51l1BV9jq1gPlzPz96kGqZaFIX/view?usp=drivesdk</a> |
| Public note |
Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
| Source of classification or shelving scheme |
Clasificación local |
| Koha item type |
Documentos solicitados |