Isolation and transcript analysis of two-component histidine kinase gene Le.nik1 in Shiitake mushroom, Lentinula edodes (Record no. 44517)

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control field 20250625140628.0
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Transcribing agency CICY
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Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-10278
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Title Isolation and transcript analysis of two-component histidine kinase gene Le.nik1 in Shiitake mushroom, Lentinula edodes
490 0# - SERIES STATEMENT
Volume/sequential designation Mycological Research, 112(1), p.108-116, 2008
520 3# - SUMMARY, ETC.
Summary, etc. Le.nik1, a two-component histidine kinase gene of Lentinula edodes, the Shiitake mushroom, was identified. The relationship between this two-component signal transduction system and mushroom development was studied. We used a modified RNA arbitrarily-primed PCR (RAP-PCR)method to isolate Le.nik1 as a differentially expressed gene during L. edodes development. We determined the 6.29 kb full-length cDNA sequence of Le.nik1. It had high sequence homology to Neurospora crassa nik1, which encoded a histidine kinase essential for development and osmotic response. In L. edodes, the expression level of Le.nik1 was highest during primordium formation and fruiting body maturation. The transcripts were localized predominantly in the developing hymenophores, or mushroom gills, which may indicate the role of a two-component signal transduction systemin cell differentiation during mushroom development. Mannitol stress influenced transcript expression of Le.nik1, suggesting that it may be involved in osmo-sensing and regulation. To our knowledge, this is the first report on the two-component system in mushrooms and the first analysis on the distribution of Le.nik1 transcript in the course of fruiting body formation and in parts of fruiting bodies.
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Topical term or geographic name entry element IN SITU RNA-RNA HYBRIDIZATION
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element MANNITOL STRESS
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element OSMOREGULATION
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element TRANSCRIPT EXPRESSION AND LOCALIZATION
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element TWO-COMPONENT SYSTEM
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Szeto, C.Y.Y.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Wong, Q.W.L.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Leung, G.S.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Kwan, H.S.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/10uJkfG51l1BV9jq1gPlzPz96kGqZaFIX/view?usp=drivesdk">https://drive.google.com/file/d/10uJkfG51l1BV9jq1gPlzPz96kGqZaFIX/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-10278 25.06.2025 25.06.2025 Documentos solicitados