Proteomics of loosely bound cell wall proteins of Arabidopsis thaliana cell suspension cultures: A critical analysis (Record no. 44569)
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| fixed length control field | 02353nam a2200337Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625140629.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-10331 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | Proteomics of loosely bound cell wall proteins of Arabidopsis thaliana cell suspension cultures: A critical analysis |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | Electrophoresis, 24, p.3421-3432, 2003 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | The complete sequencing of the Arabidopsis thaliana genome allows the use of the recently developed mass spectrometry techniques to identify the cell wall proteins (CWPs). Most proteomic approaches depend on the quality of sample preparation. Extraction of CWPs is particularly complex since the proteins may be free in the apoplast or are embedded in a polysaccharide matrix where they are retained by Van der Waals interactions, hydrogen bonds, hydrophobic or ionic interactions, or cross-linked by covalent bonds. Specific and sequential extraction procedures thus need to be developed. We report on the sequential extraction of loosely bound CWPs from living A. thaliana cells in culture. Different salts and chelating agents were used for releasing the proteins from the wall. Their effects on the extraction of CWPs and on the integrity of the plasma membrane were evaluated. Bioinformatic software was used to identify proteins and to predict their sub-cellular localization. The obtained data show that the plasma membrane of cells in culture was easily damaged by some steps of the extraction procedure, leading to the release of increasing amounts of intracellular proteins. Nevertheless, we identified fifty CWPs among which thirteen were new proteins for the cell wall. In addition, 76 |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | ARABIDOPSIS THALIANA |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | CELL SUSPENSION CULTURE |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | CELL WALL |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | MASS SPECTROMETRY |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | PROTEOMICS |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Borderies, G. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Jamet, E. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Lafitte, C. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Rossignol, M. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Jauneau, A. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Boudart, G. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Monsarrat, B. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Monsarrat, B. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Boudet, A. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Pont-Lezica, R. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1B2jBklTSDwunuqjw8WTcYqy3geLETVlE/view?usp=drivesdk">https://drive.google.com/file/d/1B2jBklTSDwunuqjw8WTcYqy3geLETVlE/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
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| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-10331 | 25.06.2025 | 25.06.2025 | Documentos solicitados |