Seed-Specific Over-Expresson of an Arabidopsis cDNA Encoding a Diacylglycerol Acyltransferase Enhances Seed Oil Content and Seed Weight (Record no. 44577)
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| fixed length control field | 02131nam a2200253Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625140629.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-10339 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | Seed-Specific Over-Expresson of an Arabidopsis cDNA Encoding a Diacylglycerol Acyltransferase Enhances Seed Oil Content and Seed Weight |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | Plant Physiology, 126, p.861-874, 2001 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | We recently reported the cloning and characterization of an Arabidopsis (ecotype Columbia)diacylglycerol acyltransferase cDNA (Zou et al., 1999)and found that in Arabidopsis mutant line AS11, an ethyl methanesulfonate-induced mutation at a locus on chromosome II designated as Tag1 consists of a 147-bp insertion in the DNA, which results in a repeat of the 81-bp exon 2 in the Tag1 cDNA. This insertion mutation is correlated with an altered seed fatty acid composition, reduced diacylglycerol acyltransferase (DGAT; EC 2.3.1.20)activity, reduced seed triacylglycerol content, and delayed seed development in the AS11 mutant. The effect of the insertion mutation on microsomal acyl-coenzyme A-dependent DGAT is examined with respect to DGAT activity and its substrate specificity in the AS11 mutant relative to wild type. We demonstrate that transformation of mutant AS11 with a single copy of the wild-type Tag1 DGAT cDNA can complement the fatty acid and reduced oil phenotype of mutant AS11. More importantly, we show for the first time that seed-specific over-expression of the DGAT cDNA in wild-type Arabidopsis enhances oil deposition and average seed weight, which are correlated with DGAT transcript levels. The DGAT activity in developing seed of transgenic lines was enhanced by 10 |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Jako, C. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Kumar, A. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Wei, Y. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Zou, J. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Barton, D.L. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Giblin, E.M. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Covello, P.S. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Covello, P.S. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1QoDkoIbUYUJz8o-vWO35b9MsR1JKfm3A/view?usp=drivesdk">https://drive.google.com/file/d/1QoDkoIbUYUJz8o-vWO35b9MsR1JKfm3A/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-10339 | 25.06.2025 | 25.06.2025 | Documentos solicitados |