The soybean Dof-type transcription factor genes, GmDof4 and GmDof11, enhance lipid content in the seeds of transgenic Arabidopsis plants (Record no. 44587)

MARC details
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fixed length control field 02548nam a2200313Ia 4500
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control field MX-MdCICY
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250625140630.0
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Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-10349
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Title The soybean Dof-type transcription factor genes, GmDof4 and GmDof11, enhance lipid content in the seeds of transgenic Arabidopsis plants
490 0# - SERIES STATEMENT
Volume/sequential designation The Plant Journal, 52, p.716-729, 2007
520 3# - SUMMARY, ETC.
Summary, etc. Soybean is one of the most important leguminous seed crops among the oil crops. Although the pathways for lipid biosynthesis have been identified, the factors that regulate the biosynthetic pathways at the transcriptional level are largely unknown. Here, we report our findings on the involvement of soybean Doftype transcription factor genes in the regulation of the lipid content in soybean seeds. We identified 28 Doftype transcription factor genes in soybean plants, and these genes displayed diverse patterns of expression in various organs. Seven flower/pod-specific genes and one constitutively expressed gene were further investigated. The proteins encoded by these seven genes were localized in the nucleus, and exhibited different abilities for transcriptional activation and DNA binding. Two genes, GmDof4 and GmDof11, were found to increase the content of total fatty acids and lipids in GmDof4 and GmDof11 transgenic Arabidopsis seeds. We also found that the 1000-seed weight was increased in the GmDof4 and GmDof11 transgenic plants. Using microarray and DNA binding analysis, we found that the two Dof-like proteins, GmDof4 and GmDof11, activated the acetyl CoA carboxylase gene and long-chain-acyl CoA synthetase gene, respectively, by direct binding to the cis-DNA elements in their promoter regions. In addition, both proteins downregulated the storage protein gene, CRA1, through direct binding. These results suggest that the two GmDof genes may augment the lipid content of soybean seeds by upregulating genes that are associated with the biosynthesis of fatty acids.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element DOF
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Topical term or geographic name entry element SEED
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element LIPID
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element SOYBEAN
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element TRANSCRIPTION FACTOR
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Wang, H.W.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Zhang, B.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Hao, Y.J.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Huang, J.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Tian, A.G.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Liao, Y.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Zhang, J.S.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Zhang, J.S.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1SxDKk9kKaSgHK06GuIZF0cnjqLaAG8tD/view?usp=drivesdk">https://drive.google.com/file/d/1SxDKk9kKaSgHK06GuIZF0cnjqLaAG8tD/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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Source of classification or shelving scheme Clasificación local
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-10349 25.06.2025 25.06.2025 Documentos solicitados