Secretome analysis of differentially induced proteins in rice suspension-cultured cells triggered by rice blast fungus and elicitor (Record no. 44611)
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| 000 -LEADER | |
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| fixed length control field | 02634nam a2200325Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625140630.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-10373 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | Secretome analysis of differentially induced proteins in rice suspension-cultured cells triggered by rice blast fungus and elicitor |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | Proteomics, 9(5), p.1302-1313, 2009 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | Secreted proteins were investigated in rice suspension-cultured cells treated with rice blast fungus Magnaporthe grisea and its elicitor using biochemical and 2-DE coupled with MSanalyses followed by their in planta mRNA expression analysis. M. grisea and elicitor successfully interacted with suspension-cultured cells and prepared secreted proteins from these cultures were essentially intracellular proteins free. Comparative 2-D gel analyses identified 21 differential protein spots due to M. grisea and/or elicitor over control. MALDI-TOF-MS and mLC-ESI-MS/MS analyses of these protein spots revealed that most of assigned proteins were involved in defense such as nine chitinases, two germin A/oxalate oxidases, five domain unknown function 26 (DUF 26)secretory proteins, and b-expansin. One chitin binding chitinase protein was isolated using chitin binding beads and strong enzymatic activity was identified in an in-gel assay. Interestingly, their protein abundance correlated well at transcript levels in elicitor-treated cultures as judged by semi-quantitative RT-PCR. Each identified differentially expressed protein group was compared at transcript levels in rice leaves inoculated with incompatible (KJ401)and compatible (KJ301)races of M. grisea. Time-course profiling revealed their inductions were stronger and earlier in incompatible than compatible interactions. Identified secreted proteins and their expression correlation at transcript level in suspension-cultured cells and also in planta suggest that suspension-cultured cells can be useful to investigate the secretome of rice blast-pathogen interactions. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | ELICITOR |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | MALDI-TOF-MS |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | M. GRISEA |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | SECRETOME |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | SUSPENSION-CULTURED CELL |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Kim, S.T. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Kang, Y.H. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Wang, Y. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Wu, J. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Park, Z.Y. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Rakwal, R. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Agrawal, G.K. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Agrawal, G.K. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Kang, K.Y. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/14edaSBmB-Ww6pGgqwzAK_g_chvXDRAdw/view?usp=drivesdk">https://drive.google.com/file/d/14edaSBmB-Ww6pGgqwzAK_g_chvXDRAdw/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
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| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-10373 | 25.06.2025 | 25.06.2025 | Documentos solicitados |