Towards crop improvement in bell pepper (Capsicum annuum L.): Transgenics (uid A::hpt II)by a tissue-culture-independent grobacterium-mediated in planta approach (Record no. 44624)

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fixed length control field 01952nam a2200289Ia 4500
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control field MX-MdCICY
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control field 20250625140630.0
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Transcribing agency CICY
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Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-10387
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Title Towards crop improvement in bell pepper (Capsicum annuum L.): Transgenics (uid A::hpt II)by a tissue-culture-independent grobacterium-mediated in planta approach
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Volume/sequential designation Scientia Horticulturae, 119(4), p.362-370, 2009
520 3# - SUMMARY, ETC.
Summary, etc. Agrobacterium tumefaciens-mediated transformation has been one of the methods used to generate transgenic plants in bell pepper. An alternate transformation method that avoids/minimizes tissue culture would be beneficial for the improvement of bell pepper due to its recalcitrant nature. In this report, transgenic bell pepper plants have been developed by a tissue-culture-independent A. tumefaciens-mediated in planta transformation procedure. In the present study, two open pollinated varieties viz., Arka Gaurav and Arka Mohini were used for transformation. Agrobacterium train EHA105 harboring the binary vector pCAMBIA1301 that carries the genes for b-glucuronidase (uid A)and hygromycin phosphotransferase II (hpt II)was used for transformation. GUS histochemical analysis of T0 and T1 plants at various stages of growth followed by molecular analysis using PCR, Southern analysis and RT-PCR allowed selection of transgenics. The method resulted in 17.8
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element CAPSICUM ANNUUM
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Topical term or geographic name entry element BELL PEPPER
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element IN PLANTA TRANSFORMATION
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element AGROBACTERIUM
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Topical term or geographic name entry element MARKER GENES
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element TRANSGENICS
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Kumar, A.M.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Reddy, K.N.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Sreevathsa, R.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Ganeshan, G.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Udayakumar, M.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1q1NWUP7ESgFDUG4peu8X5Qxl5cuPtUcF/view?usp=drivesdk">https://drive.google.com/file/d/1q1NWUP7ESgFDUG4peu8X5Qxl5cuPtUcF/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-10387 25.06.2025 25.06.2025 Documentos solicitados