Detection of exo-P-1,3-glucanase activity in polyacrylamide gels after electrophoresis under denaturing or nondenaturing conditions (Record no. 44640)
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| fixed length control field | 01577nam a2200181Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625140631.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-10405 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | Detection of exo-P-1,3-glucanase activity in polyacrylamide gels after electrophoresis under denaturing or nondenaturing conditions |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | Electrophoresis, 15(1), p.903-906, 1994 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | A method for the visualization of exo-P-1,3-glucanase activity in polyacryl- amide gels is presented. The procedure consists of the enzyme reaction in the gel with the substrate a-naphthylglucopyranoside, and a subsequent staining of the obtained a-naphthol with dyes Fast Red B, or Fast Blue BB, respec- tively. A mixture of exoglucanases produced by the fungus Polyporus squa- moms was used for the optimization of the method. The procedure is applic- able for the standard Laemmli discontinuous electrophoresis system, even in the presence of sodium dodecyl sulfate, as well as for electrophoresis in linear gradients of the polyacrylamide concentration. The staining method was used for the analysis of exoglucanases secreted by several yeast genera. All yeasts tested produced two types of exoglucanases, a high molecular mass species heterogeneous in size, and one or two smaller homogeneous enzymes. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Vargic, T. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Mrsa, V. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1NHfmWT9kOZLe9O1rsTz_2Ah530NFnHXW/view?usp=drivesdk">https://drive.google.com/file/d/1NHfmWT9kOZLe9O1rsTz_2Ah530NFnHXW/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
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| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-10405 | 25.06.2025 | 25.06.2025 | Documentos solicitados |