Recombination and expression of classical swine fever virus (CSFV)structural protein E2 gene in Chlamydomonas reinhardtii chroloplasts (Record no. 44689)

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fixed length control field 01702nam a2200325Ia 4500
003 - CONTROL NUMBER IDENTIFIER
control field MX-MdCICY
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250625140632.0
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Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-10457
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245 10 - TITLE STATEMENT
Title Recombination and expression of classical swine fever virus (CSFV)structural protein E2 gene in Chlamydomonas reinhardtii chroloplasts
490 0# - SERIES STATEMENT
Volume/sequential designation Colloids and Surfaces B: Biointerfaces, 55(1), p.26-30, 2007
520 3# - SUMMARY, ETC.
Summary, etc. The expression of classical swine fever virus (CSFV)structural protein E2 in different vectors, which has been shown to carry critical epitopes, has been established. Here, we reported a Chlamydomonas reinhardtii chloroplast expression vector, P64E2, containing classical swine fever virus structural protein E2 gene, which was constructed and transferred to C. reinhardtii by biolistic bombardment method. The transformants were identified by PCR, Southern blotting, Western blotting after selecting on resistant media. ELISA quantification assay showed that the expressed E2 protein accumulated up to 1.5-2
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element CLASSICAL SWINE FEVER VIRUS (CSFV)
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element E2 GENE
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element CHLAMYDOMONAS REINHARDTII
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element CHLOROPLAST TRANSFORMATION
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element ANTI-DISEASE PROTEIN
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element BIOINTERFACE
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element PLANT GENE ENGINEERING
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name He, D.M.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Qian, K.X.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Shen, G.F.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Zhang, Z.F.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Lie, Y.N.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Sud, Z.L.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Shao, H.B.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1jDsK7jMNxQoyc7fEkp-oPz0uhHnVpzvX/view?usp=drivesdk">https://drive.google.com/file/d/1jDsK7jMNxQoyc7fEkp-oPz0uhHnVpzvX/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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Source of classification or shelving scheme Clasificación local
Koha item type Documentos solicitados
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-10457 25.06.2025 25.06.2025 Documentos solicitados