Cloning and characterization of a plastidic glycerol 3-phosphate dehydrogenase cDNA from Dunaliella salina (Record no. 44731)

MARC details
000 -LEADER
fixed length control field 02154nam a2200313Ia 4500
003 - CONTROL NUMBER IDENTIFIER
control field MX-MdCICY
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250625140632.0
040 ## - CATALOGING SOURCE
Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-10499
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 250602s9999 xx |||||s2 |||| ||und|d
245 10 - TITLE STATEMENT
Title Cloning and characterization of a plastidic glycerol 3-phosphate dehydrogenase cDNA from Dunaliella salina
490 0# - SERIES STATEMENT
Volume/sequential designation Journal of Plant Physiology, 164(2), p.214-220, 2007
520 3# - SUMMARY, ETC.
Summary, etc. A cDNA encoding a nicotinamide adenine dinucleotide (NAD+)-dependent glycerol 3-phosphate dehydrogenase (GPDH)has been cloned by rapid amplification of cDNA ends from Dunaliella salina. The cDNA is 3032 base pairs long with an open reading frame encoding a polypeptide of 701 amino acids. The polypeptide shows high homology with published NAD+-dependent GPDHs and has at its N-terminal a chloroplast targeting sequence. RNA gel blot analysis was performed to study GPDH gene expression under different conditions, and changes of the glycerol content were monitored. The results indicate that the cDNA may encode an osmoregulated isoform primarily involved in glycerol synthesis. The 701-amino-acid polypeptide is about 300 amino acids longer than previously reported plant NAD+-dependent GPDHs. This 300-amino-acid fragment has a phosphoserine phosphatase domain. We suggest that the phosphoserine phosphatase domain functions as glycerol 3-phosphatase and that, consequently, NAD+-dependent GPDH from D. salina can catalyze the step from dihydroxyacetone phosphate to glycerol directly. This is unique and a possible explanation for the fast glycerol synthesis found in D. salina
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element DUNALIELLA SALINA
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element GLYCEROL
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element GLYCEROL 3-PHOSPHATASE
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element GLYCEROL 3-PHOSPHATE
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element DEHYDROGENASE
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element OSMOTIC STRESS
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name He, Q.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Qiao, D.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Bai, L.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Zhang, Q.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Yang, W.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Li, Q.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Cao, Y.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1xeG2iPOE_8Mh8oJsv1SuTXsUYrpy-mTA/view?usp=drivesdk">https://drive.google.com/file/d/1xeG2iPOE_8Mh8oJsv1SuTXsUYrpy-mTA/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Source of classification or shelving scheme Clasificación local
Koha item type Documentos solicitados
Holdings
Lost status Source of classification or shelving scheme Damaged status Not for loan Collection Home library Current library Shelving location Date acquired Total checkouts Full call number Date last seen Price effective from Koha item type
  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-10499 25.06.2025 25.06.2025 Documentos solicitados