Selective Isolation of Newly Synthesized Mammalian mRNA after in Vivo Labeling with 4-Thiouridine or 6-Thioguanosine (Record no. 44759)
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| fixed length control field | 02312nam a2200277Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625140633.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-10527 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | Selective Isolation of Newly Synthesized Mammalian mRNA after in Vivo Labeling with 4-Thiouridine or 6-Thioguanosine |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | Analytical BioChemistry, 171(1), p.166-172, 1988 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | Newly synthesized mRNA from hamster cells was labeled in vivo with the thionucleoside analogs 4-thiouridine (4-TU)and 6-thioguanosine (6-TG). The thio-substituted RNA was selectively recovered by Affi-Gel 501 phenylmercury affinity chromatography. Following a l-h labeling period, enrichment for newly transcribed RNA after a single round of chromatography ranged between lo- and 15-fold when compared with total RNA. Exposure of CHO UrdA cells, a uridine auxotrophic line, to 50 pM 4-TLJ allowed for optimal recovery of newly transcribed RNA. Increasing the concentration of 4-TU to 100 pM or labeling with 6-TG at concentrations of 3 FM or greater resulted in similar recoveries from uridine-prototrophic hamster cell lines. For shorter term labeling, exposure of prototrophic cells to 500 fiM 4-TU or 100 pM 6-TG for 15 min allowed newly synthesized RNA to be selectively recovered. As a specific test case, enrichment for histone H3.2 mRNA was analyzed after hamster cells were labeled with 4-TU under conditions in which the gene was highly transcriptionally active. Northern blot analysis and the specific activity of thio-substituted RNA revealed a 15-fold enrichment when compared to total RNA. In vivo labeling of cellular RNA with 4-TU or 6-TG should provide a useful method for studying inducible gene expression and for isolating and cloning specific mRNAs from mammalian cells. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | CHROMATOGRAPHY |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | NUCLEIC ACIDS |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | AFFINITY CHROMATOGRAPHY |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | RNA ISOLATION |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | HISTONE MRNA |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | 4-THIOURIDINE |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | 6-THIOGUANOSINE |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Woodford, T. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Schlegel, R. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Pardee, A.B. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1GlctxThmfEesJjvsgmJ6nq2vmYPct8DZ/view?usp=drivesdk">https://drive.google.com/file/d/1GlctxThmfEesJjvsgmJ6nq2vmYPct8DZ/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
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| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-10527 | 25.06.2025 | 25.06.2025 | Documentos solicitados |