Discovery of Pathogenicity Genes in the Crucifer Anthracnose Fungus Colletotrichum higginsianum, Using Random Insertional Mutagenesis (Record no. 44778)

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control field 20250625140633.0
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Transcribing agency CICY
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Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-10546
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Title Discovery of Pathogenicity Genes in the Crucifer Anthracnose Fungus Colletotrichum higginsianum, Using Random Insertional Mutagenesis
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Volume/sequential designation Molecular Plant-Microbe Interactions, 22(2), p.143-156, 2009
520 3# - SUMMARY, ETC.
Summary, etc. Agrobacterium tumefaciens-mediated transformation (ATMT)was used for random insertional mutagenesis to identify pathogenicity genes in the hemibiotrophic fungus Colletotrichum higginsianum. A high-throughput primary infection assay on Arabidopsis thaliana seedlings allowed the rapid screening of 8,850 transformants. Forty mutants showing reproducible pathogenicity defects on Arabidopsis and Brassica plants were obtained, and their infection phenotypes were characterized microscopically. Six mutants were impaired in appressorial melanization, fifteen had reduced penetration ability, 14 induced host papillae or hypersensitive cell death, and five were affected in the transition from biotrophy to necrotrophy. Southern blot analysis showed 58
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Personal name Huser, A.
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Personal name Takahara, H.
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Personal name Schmalenbach, W.
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Personal name O'Connell, R.
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Uniform Resource Identifier <a href="https://drive.google.com/file/d/1CsL8Upt8HrpAk0CXsZnOxPM4n2lydOy3/view?usp=drivesdk">https://drive.google.com/file/d/1CsL8Upt8HrpAk0CXsZnOxPM4n2lydOy3/view?usp=drivesdk</a>
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Source of classification or shelving scheme Clasificación local
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-10546 25.06.2025 25.06.2025 Documentos solicitados