Directing Vanillin Production From Ferulic Acid by Increased Acetyl-CoA Consumption in Recombinant Escherichia coli (Record no. 44798)
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| fixed length control field | 02136nam a2200265Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625140634.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-10566 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | Directing Vanillin Production From Ferulic Acid by Increased Acetyl-CoA Consumption in Recombinant Escherichia coli |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | Biotechnology and BioEngineering, 102(1), p.200-208, 2009 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | The amplification of gltA gene encoding citrate synthase of TCA cycle was required for the efficient conversion of acetyl-CoA, generated during vanillin production from ferulic acid, to CoA, which is essential for vanillin production. Vanillin of 1.98 g/L was produced from the E. coli DH5a (pTAHEF-gltA)with gltA amplification in 48 h of culture at 3.0 g/L of ferulic acid, which was about twofold higher than the vanillin production of 0.91 g/L obtained by the E. coli DH5a (pTAHEF)without gltA amplification. The icdA gene encoding isocitrate dehydrogenase of TCA cycle was deleted to make the vanillin producing E. coli utilize glyoxylate bypass which enables more efficient conversion of acetyl-CoA to CoA in comparison with TCA cycle. The production of vanillin by the icdA null mutant of E. coli BW25113 harboring pTAHEF was enhanced by 2.6 times. The gltA amplification of the glyoxylate bypass in the icdA null mutant remarkably increased the production rate of vanillin with a little increase in the amount of vanillin production. The real synergistic effect of gltA amplification and icdA deletion was observed with use of XAD-2 resin reducing the toxicity of vanillin produced during culture. Vanillin of 5.14 g/L was produced in 24 h of the culture with molar conversion yield of 86.6 |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Lee, E. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Yoon, S. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Das, A. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Lee, S. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Li, C. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Kim, J. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Choi, M. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Choi, M. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Kim, S. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1KFa4zEOMd6OPIsW5sh6phEsd_re19OG6/view?usp=drivesdk">https://drive.google.com/file/d/1KFa4zEOMd6OPIsW5sh6phEsd_re19OG6/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
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| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-10566 | 25.06.2025 | 25.06.2025 | Documentos solicitados |