An Efficient Method for the Extraction of High-Quality Fungal Total RNA to Study the Mycosphaerella fijiensis-Musa spp. Interaction (Record no. 44912)
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| fixed length control field | 01864nam a2200253Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625140636.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-10680 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | An Efficient Method for the Extraction of High-Quality Fungal Total RNA to Study the Mycosphaerella fijiensis-Musa spp. Interaction |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | Mol. Biotechnol., 40(3), p.299-305, 2008 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | Efficient RNA isolation is a prerequisite for gene expression studies and it has an increasingly important role in the study of plant-fungal pathogen interactions. However, RNA isolation is difficult in filamentous fungi. These organisms are notorious for their rigid cell walls and the presence of high levels of carbohydrates, excreted from the fungal cells during submerged growth, which interferes with the extraction procedures. Although many commercial kits are already available for RNA isolation, they do not provide, in most cases, enough amount of pure RNA to be used in upstream applications. In the present work, we propose an easy and efficient protocol for isolating total RNA from the filamentous fungus Mycosphaerella fijiensis, the most important foliar pathogen of Musa spp. varieties worldwide. In addition, we applied the proposed protocol to the isolation of total RNA from banana leaves infected with the pathogen. Our methodology was developed based on the SDS method with modifications |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Sánchez-Rodríguez, A. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Portal, O. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Rojas, L.E. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Ocaña, B. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Mendoza, M. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Acosta, M. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Jiménez, E. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Jiménez, E. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1kbP4ZHKt3e1uPb2Z1e6cZnGt805A0jo6/view?usp=drivesdk">https://drive.google.com/file/d/1kbP4ZHKt3e1uPb2Z1e6cZnGt805A0jo6/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-10680 | 25.06.2025 | 25.06.2025 | Documentos solicitados |