Reevaluation of the Phenol-Sulfuric Acid Reaction for the Estimation of Hexoses and Pentoses (Record no. 44966)

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Transcribing agency CICY
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Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-10734
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245 10 - TITLE STATEMENT
Title Reevaluation of the Phenol-Sulfuric Acid Reaction for the Estimation of Hexoses and Pentoses
490 0# - SERIES STATEMENT
Volume/sequential designation Analytical BioChemistry, 181, p.18-22, 1989
520 3# - SUMMARY, ETC.
Summary, etc. Evidence is provided to show that in the conventional phenol-sulfuric acid reaction procedure, phenol underwent sulfonation in situ and the phenolsulfonic acid formed decreased the color intensity for hydroxymethyl furfural (HMF), furfural, and many hexoses and pentoses tested. A modified method is described to overcome this problem in which phenol was added after the dehydration of carbohydrates by sulfuric acid and after cooling the system. The color intensity around 476-485 nm for different compounds was fairly proportional to the amount of furfural derivatives (absorption at 310-320 nm)formed from the sugars in the modified method unlike in the conventional procedure. The studies also show that for condensation of HMF derivatives with phenol, heat is not necessary. The color intensity in the modified method also increased compared to that in the conventional method. The increase in the modified method compared to that in the conventional method was 6.0-fold for furfural, 9.1-fold for hydroxymethyl furfural, 3.7-fold for fructose, 2.3-fold for xylose, and 2.0-fold for glucose and arabinose. The possible reasons for this differential increase are discussed.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Rao, P.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Pattabiraman, T.N.
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Uniform Resource Identifier <a href="https://drive.google.com/file/d/1O6MABdZv4hjMJ-xP7H_tgOOOLHgaxff_/view?usp=drivesdk">https://drive.google.com/file/d/1O6MABdZv4hjMJ-xP7H_tgOOOLHgaxff_/view?usp=drivesdk</a>
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-10734 25.06.2025 25.06.2025 Documentos solicitados