Complexation of Proteins with a Strong Polyanion in an Aqueous Salt-free System (Record no. 44979)
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| fixed length control field | 02112nam a2200229Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625140637.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-10747 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | Complexation of Proteins with a Strong Polyanion in an Aqueous Salt-free System |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | Langmuir, 12, p.6295-6303, 1996 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | We studied the formation in a salt-free solution of complexes between potassium poly(vinyl alcohol)sulfate (KPVS), a strong polyelectrolyte, and proteins with known amino-acid sequences: papain, human serum albumin, lysozyme, ribonuclease, trypsin, and pepsin. Turbidimetric titration, together with quasielastic light scattering (QELS), static light scattering (SLS), and electrophoretic light scattering, was performed at pH 2, in order to completely protonate the basic groups of proteins and so facilitate the determination of their total cationic charge. Even at pH 2, no protein unfolding was observed in all the samples within the precision of QELS. Through complexation with KPVS at pH 2, electrically neutral protein-polyelectrolyte complexes (PPCs)with a uniform size were formed from all the proteins, other than pepsin. Such PPCs can be regarded as aggregates of an intrapolymer complex consisting of a KPVS chain with the bound protein molecules, the formation of which occurs via the stoichiometric neutralization of the polyion charges with the opposite charges of proteins. After most of the proteins have formed PPCs, further addition of KPVS led to the association of the aggregated intrapolymer PPCs to form high-ordered aggregates. However, a decrease in the protein charges due to the characteristics of proteins or pH level appears to alter the nature of the PPC formed. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Tsuboi, A. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Izumi, T. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Hirata, M. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Xia, J. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Dubin, P.L. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Kokufuta, E. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1sJ3PAhPy73_j38sRBGF9Wyz55snD0G0f/view?usp=drivesdk">https://drive.google.com/file/d/1sJ3PAhPy73_j38sRBGF9Wyz55snD0G0f/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-10747 | 25.06.2025 | 25.06.2025 | Documentos solicitados |