MARC details
| 000 -LEADER |
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| fixed length control field |
02424nam a2200277Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
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| control field |
MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20250625140637.0 |
| 040 ## - CATALOGING SOURCE |
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| Transcribing agency |
CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
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| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
B-10748 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT |
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| Title |
Light Scattering, CD, and Ligand Binding Studies of Ferrihemoglobin-Polyelectrolyte Complexes |
| 490 0# - SERIES STATEMENT |
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| Volume/sequential designation |
BioPolymers, 50, p.153-161, 1999 |
| 520 3# - SUMMARY, ETC. |
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| Summary, etc. |
Quasi-elastic light scattering (QELS), electrophoretic light scattering (ELS), CD spectroscopy, and azide binding titrations were used to study the complexation at pH 6.8 between ferrihemoglobin and three polyelectrolytes that varied in charge density and sign. Both QELS and ELS show that the structure of the soluble complex formed between ferrihemoglobin and poly(diallyldimethylammonium chloride)[PDADMAC]varies with protein concentration. At fixed 1.0 mg/mL polyelectrolyte concentration, protein addition increases complex size and decreases complex mobility in a tightly correlated manner. At 1.0 mg/mL or greater protein concentration, a stable complex is formed between one polyelectrolyte chain and many protein molecules (i.e., an intrapolymer complex)with apparent diameter approximately 2.5 times that of the protein-free polyelectrolyte. Under conditions of excess polyelectrolyte, each of the three ferrihemoglobin-polyelectrolyte solutions exhibits a single diffusion mode in QELS, which indicates that all protein molecules are complexed. CD spectra suggest little or no structural disruption of ferrihemoglobin upon complexation. Azide binding to the ferrihemoglobin-poly(2-acrylamide-2-methylpropanesulfonate)[PAMPS]complex is substantially altered relative to the polyelectrolyte-free protein, but minimal change is induced by complexation with an AMPS-based copolymer of reduced linear charge density. The change in azide binding induced by PDADMAC is intermediate between that of PAMPS and its copolymer |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
HEMOGLOBIN-POLYELECTROLYTE COMPLEXES |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
PROTEIN-POLYELECTROLYTE COMPLEXES |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
LIGHT SCATTERING |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
CD |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
LIGAND BINDING |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Xia, J. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Dubin, P.L. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Kokufuta, E. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Havel, H. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Muhoberac, B.B. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS |
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| Uniform Resource Identifier |
<a href="https://drive.google.com/file/d/11Gzbfp0fHr_nl_0V_rK3iNdyTxJAeCgn/view?usp=drivesdk">https://drive.google.com/file/d/11Gzbfp0fHr_nl_0V_rK3iNdyTxJAeCgn/view?usp=drivesdk</a> |
| Public note |
Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
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| Source of classification or shelving scheme |
Clasificación local |
| Koha item type |
Documentos solicitados |
