Elucidation of the Phosphate Binding Mode of DING Proteins Revealed by Subangstrom X-ray Crystallography (Record no. 45004)

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control field 20250625140637.0
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Transcribing agency CICY
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Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-10772
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Title Elucidation of the Phosphate Binding Mode of DING Proteins Revealed by Subangstrom X-ray Crystallography
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Volume/sequential designation J. Am. Chem. Soc., 22(131), p.7879-7886, 2009
520 3# - SUMMARY, ETC.
Summary, etc. PfluDING is a bacterial protein isolated from Pseudomonas fluorescens that belongs to the DING protein family, which is ubiquitous in eukaryotes and extends to prokaryotes. DING proteins and PfluDING have very similar topologies to phosphate Solute Binding Proteins (SBPs). The three-dimensional structure of PfluDING was obtained at subangstrom resolution (0.88 and 0.98 Å)at two different pH's (4.5 and 8.5), allowing us to discuss the hydrogen bond network that sequesters the phosphate ion in the binding site. From this high resolution data, we experimentally elucidated the molecular basis of phosphate binding in phosphate SBPs. The phosphate ion is tightly bound to the protein via 12 hydrogen bonds between phosphate oxygen atoms and OH and NH groups of the protein. The proton on one oxygen atom of the phosphate dianion forms a 2.5 Å low barrier hydrogen bond with an aspartate, with the energy released by forming this strong bond ensuring the specificity for the dianion even at pH 4.5. In particular, contrary to previous theories on phosphate SBPs, accurate electrostatic potential calculations show that the binding cleft is positively charged. PfluDING structures reveal that only dibasic phosphate binds to the protein at both acidic and basic phosphate, suggesting that the protein binding site environment stabilizes the HPO4 2- form of phosphate.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Liebschner, D.
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Personal name Elias, M.
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Personal name Moniot, S.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Fournier, B.
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Personal name Scott, K.
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Personal name Jelsch, C.
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Personal name Guillot, B.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Guillot, B.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Chabriere, E.
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Uniform Resource Identifier <a href="https://drive.google.com/file/d/1vNc8z15zYNCtAm9kfke9ZCX1elvNawpC/view?usp=drivesdk">https://drive.google.com/file/d/1vNc8z15zYNCtAm9kfke9ZCX1elvNawpC/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-10772 25.06.2025 25.06.2025 Documentos solicitados