A silver staining procedure for nucleic acids in polyacrylamide gels without fixation and pretreatment (Record no. 45008)

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fixed length control field 01380nam a2200241Ia 4500
003 - CONTROL NUMBER IDENTIFIER
control field MX-MdCICY
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250625140638.0
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Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-10776
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245 10 - TITLE STATEMENT
Title A silver staining procedure for nucleic acids in polyacrylamide gels without fixation and pretreatment
490 0# - SERIES STATEMENT
Volume/sequential designation Analytical BioChemistry, 391(1), p.77-79, 2009
520 3# - SUMMARY, ETC.
Summary, etc. Silver staining of nucleic acid has been widely used in molecular marker analysis such as simple sequence repeat (SSR), single-strand conformation polymorphism (SSCP), and amplified fragment-length polymorphism (AFLP). Many alternatives to silver staining methods have been described, but these methods are not efficient or cost-effective. Here we report a silver staining method that requires less than 10 min for one gel and can save chemicals as well. It has a detection limit of approximately 5.6 pg of DNA/mm2 in nondenaturing polyacrylamide gels and 12.8 pg/mm2 in denaturing polyacrylamide gels.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name An, Z.W.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Xie, L.L.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Cheng, H.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Zhou, Y.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Zhang, Q.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name He, X.G.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Huang, H.S.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1ikCCnAkDuaZ9BTzSkWlHJurbYiowYmdd/view?usp=drivesdk">https://drive.google.com/file/d/1ikCCnAkDuaZ9BTzSkWlHJurbYiowYmdd/view?usp=drivesdk</a>
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Source of classification or shelving scheme Clasificación local
Koha item type Documentos solicitados
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-10776 25.06.2025 25.06.2025 Documentos solicitados