Quantification of PCR products by phosphate measurement (Record no. 45015)

MARC details
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fixed length control field 01677nam a2200229Ia 4500
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control field MX-MdCICY
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250625140638.0
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Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-10783
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245 10 - TITLE STATEMENT
Title Quantification of PCR products by phosphate measurement
490 0# - SERIES STATEMENT
Volume/sequential designation Analytical BioChemistry, 384(2), p.305-311, 2009
520 3# - SUMMARY, ETC.
Summary, etc. Various techniques for quantification of PCR are available. Most frequently, the densitometric intensities of ethidium bromide- stained PCR roducts separated in gels are compared after normalizing to the levels of housekeeping gene products such as b-actin. More precise, but extremely time consuming, is the technique of competitive PCR. Newer methods, such as tracking amplification in real-time, have high start-up and maintenance costs (e.g., TaqMan, Applied Biosystems; LightCycler, Roche; I-Cycler, Bio-Rad). Here, I describe an alternative, simple technique to quantify PCR products by determining the entire phosphate released during PCR.The method can be performed using common laboratory equipment, and the reagents needed are extremely cheap. The method is validated by measuring the induction of inducible nitric oxide synthase gene expression in cell culture and comparing the results with data obtained by LightCycler experiments and RNase protection assays.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element PCR
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element RT-PCR
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element PHOSPHATE
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element QUANTIFICATION
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element MOLYBDATE
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Arneth, B.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1VfhNJsq-hMtfx-z-XTYGEhpHh_27nFGx/view?usp=drivesdk">https://drive.google.com/file/d/1VfhNJsq-hMtfx-z-XTYGEhpHh_27nFGx/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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Source of classification or shelving scheme Clasificación local
Koha item type Documentos solicitados
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-10783 25.06.2025 25.06.2025 Documentos solicitados