Changes in morphology and biochemical indices in browning callus derived from Jatropha curcas hypocotyls (Record no. 45085)

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003 - CONTROL NUMBER IDENTIFIER
control field MX-MdCICY
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250625140639.0
040 ## - CATALOGING SOURCE
Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-10854
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245 10 - TITLE STATEMENT
Title Changes in morphology and biochemical indices in browning callus derived from Jatropha curcas hypocotyls
490 0# - SERIES STATEMENT
Volume/sequential designation Plant Cell, Tissue and Organ Culture , 98, p.11-17, 2009
520 3# - SUMMARY, ETC.
Summary, etc. Callus browning is a typical feature of callus cultures derived from the hypocotyl of Jatropha curcas. Brown callus results in decreased regenerative ability, poor growth and even death. In this study, we investigated the effect of browning on callus morphology and biochemical indices. Light microscopy and scanning electron microscopy showed striking differences in callus morphology. During browning, chlorophylls and carotenoids concentrations decreased steadily. Polyphenol oxidase (PPO)and peroxidase (POD)enzymatic activities patterns were similar during callus culture with a higher activity level at week 3 compared to week 2 or later weeks. Grey relation degree analysis indicated that PPO played a more important role than POD in enzymatic callus browning. Polyacrylamide gel electrophoresis results showed differences between browning and non-browning callus. Gas chromatography- mass spectrometry results showed that saturated and unsaturated fatty acid quantities differed significantly but there was little difference in fatty acid composition between non-browning and browning callus. Differences in 17, 18.4 and 25 kDa protein concentrations were also observed in browning and non-browning callus using sodium dodecyl sulfate-polyacrylamide gel electrophoresis.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element JATROPHA CURCAS
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element BROWNING
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element CALLUS
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element PPO
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element POD
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element TISSUE CULTURE
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name He, Y.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Guo, X.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Lu, R.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Niu, B.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Pasapula, V.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Hou, P.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Cai, F.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Cai, F.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Chen, F.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1ZX6RrOGIUhVwROdQtcD4PIcXCrvD0EoF/view?usp=drivesdk">https://drive.google.com/file/d/1ZX6RrOGIUhVwROdQtcD4PIcXCrvD0EoF/view?usp=drivesdk</a>
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Source of classification or shelving scheme Clasificación local
Koha item type Documentos solicitados
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Lost status Source of classification or shelving scheme Damaged status Not for loan Collection Home library Current library Shelving location Date acquired Total checkouts Full call number Date last seen Price effective from Koha item type
  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-10854 25.06.2025 25.06.2025 Documentos solicitados