Group-Specific Primer and Probe Sets to Detect Methanogenic Communities Using Quantitative Real-Time Polymerase Chain Reaction (Record no. 45099)

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control field 20250625140639.0
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Transcribing agency CICY
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Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-10868
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Title Group-Specific Primer and Probe Sets to Detect Methanogenic Communities Using Quantitative Real-Time Polymerase Chain Reaction
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Volume/sequential designation Biotechnology and BioEngineering, 89, p.670-679, 2005
520 3# - SUMMARY, ETC.
Summary, etc. Real-time polymerase chain reaction (PCR)is a highly sensitive method that can be used for the detection and quantification of microbial populations without cultivating them in anaerobic processes and environmental samples. This work was conducted to design primer and probe sets for the detection of methanogens using a real-time PCR with the TaqMan system. Six group-specific methanogenic primer and probe sets were designed. These sets separately detect four orders (Methanococcales, Methanobacteriales, Methanomicrobiales, and Methanosarcinales)along with two families (Methanosarcinaceae and Methanosaetaceae)of the order Methanosarcinales. We also designed the universal primer and probe sets that specifically detect the 16S rDNA of prokaryotes and of the domain Bacteria and Archaea, and which are fully compatible with the TaqMan real-time PCR system. Target-group specificity of each primer and probe set was empirically verified by testing DNA isolated from 28 archaeal cultures and by analyzing potential false results. In general, each primer and probe set was very specific to the target group. The primer and probe sets designed in this study can be used to detect and quantify the order-level (family-level in the case of Methanosarcinales)methanogenic groups in anaerobic biological processes and various environments.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element ANAEROBIC DIGESTION
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element METHANOGEN
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Topical term or geographic name entry element MICROBIAL COMMUNITY
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element PRIMER AND PROBE SET
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element REAL-TIME QUANTITATIVE POLYMERASE CHAIN REACTION
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Yu, Y.
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Personal name Lee, C.
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Personal name Kim, J.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Hwang, S.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/10JedEYX0bYmpKeuRwQFHP61DAK0tp1_v/view?usp=drivesdk">https://drive.google.com/file/d/10JedEYX0bYmpKeuRwQFHP61DAK0tp1_v/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-10868 25.06.2025 25.06.2025 Documentos solicitados